甘蓝型矮秆油菜DW871激素敏感性及矮化突变机制的初步分析  

Preliminary analysis of hormone sensitivity and dwarf mutation mechanism of dwarf mutant DW871 in Brassica napus L.

作　　者：王敏 黄莎[2] 张瑞茂 李杨[2] 王芳 杨元雨 王转转 李超 WANG Min;HUANG Sha;ZHANG Rui-mao;LI Yang;WANG Fang;YANG Yuan-yu;WANG Zhuan-zhuan;LI Chao(School of Life Science,Guizhou Normal University,Guiyang 550025,China;Guizhou Academy of Agricultural Sciences/Oil Research Institute/Rape Research Institute/Guizhou Plant Conservation Technology Application Engineering Research Center,Guiyang 550006,China;Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),College of Life Sciences/Institute of Agro-Bioengineering,Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州师范大学生命科学学院,贵州贵阳550025 [2]贵州省农业科学院/油料研究所/油菜研究所/植物保育技术应用工程研究中心,贵州贵阳550006 [3]贵州大学生命科学学院/农业生物工程研究院,山地植物资源保护与种质创制教育部重点实验室,贵州贵阳550025

出　　处：《中国油料作物学报》2024年第4期772-780,共9页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(32060464);贵州省人才基地(RCJD2018-14);贵州省高层次(十层次)创新型人才培养(黔科合平台人才[2016]4003号)。

摘　　要：本研究以甘蓝型矮秆油菜DW871及同源正常高秆油菜HW871为材料,通过光暗形态分析、对激素的敏感性分析及内源激素含量测定,初步探究DW871矮化突变类型。结果表明,DW871的光暗形态表型正常,下胚轴对外源独脚金内酯、赤霉素及油菜素内酯敏感性弱于HW871,IAA对DW871下胚轴伸长影响最小,且抽薹期外施激素后DW871的矮化表型未发生变化;测定了内源激素含量,结果DW871的生长素和赤霉素含量高于HW871,油菜素内酯与HW871无差异,独脚金内酯含量低于HW871。通过光暗形态建成与激素敏感性分析,初步推测甘蓝型矮秆油菜DW871矮化机制可能与激素缺陷关系不大。In this study,the dwarf mutant DW871 and high-stem rapeseed homologous HW871 were used to investigate the type of dwarf mutation by light and dark morphological analysis,hormone sensitivity analysis and determination of endogenous hormonal content.The results showed that DW871 had a normal light-dark phenotype,and hypocotyles and stems were sensitive to exogenous strigolactone,gibberellin,and brassinolide,insensitive to exogenous auxin,and the dwarf phenotype of DW871 could not be restored after bolting during external hormone administration.The determination of the endogenous hormones content showed that the auxin and gibberellin content in DW871 was higher than in HW871,and the content of strigolactones was lower than that in HW871,the brassinolide content was no different from that in HW871.In conclusion,we speculated that the dwarf of Brassica napus DW871 could not be related to hormone defects.

关 键 词：甘蓝型油菜 DW871 油菜素内酯 赤霉素 生长素 独脚金内酯 

分 类 号：S565.4[农业科学—作物学]