不同空气暴露时间对波纹巴非蛤非特异性免疫功能的影响  

Effect of different air exposure time on non-specific immune function of Paphia undulata

作　　者：刘芸光 陈志[2] 蒋奕雄[2] 钟国龙 林岗[1] 林位琅 LIU Yunguang;CHEN Zhi;JIANG Yixiong;ZHONG Guolong;LIN Gang;LIN Weilang(College of Life Science,Fujian Normal University,Fuzhou 350117,China;Freshwater Fisheries Research Institute of Fujian,Fuzhou 350025,China;Fujian Fishery Technical Extension Center,Fuzhou 350025,China)

机构地区：[1]福建师范大学生命科学学院,福建福州350117 [2]福建省淡水水产研究所,福建福州350025 [3]福建省水产技术推广总站,福建福州350025

出　　处：《渔业研究》2024年第4期331-337,共7页Journal of Fisheries Research

基　　金：2023年福建省海洋与渔业高质量发展专项(FJHY-YYKJ-2023-2-1)。

摘　　要：【目的】探究不同空气暴露时间对海产贝类波纹巴非蛤(Paphia undulata)免疫相关指标的影响。【方法】分别将波纹巴非蛤置于空气中暴露0(对照组)、6、12和24 h后恢复海水培养,在恢复海水培养0、2、6、12、24 h时,取波纹巴非蛤斧足组织进行低温匀浆后,测定其酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、丙二醛(MDA)、一氧化氮(NO)等指标【结果】24 h以内的空气暴露时间对波纹巴非蛤水解酶(ACP、AKP)活力无显著的影响(P>0.05),但对抗氧化酶(SOD、CAT)活力和氧自由基(MDA、NO)含量有较大的影响。空气暴露对波纹巴非蛤抗氧化酶活力具有一定的抑制作用,且空气暴露时间越长,波纹巴非蛤SOD和CAT活力的变化波动越大,受到的氧化损伤越严重。空气暴露时间越长,波纹巴非蛤MDA含量也越高,膜损伤越严重;NO含量呈先上升后下降的趋势,可能与短时间空气暴露产生的大量NO对机体起到保护作用有关。【结论】在24 h以内,空气暴露时间越长,波纹巴非蛤抗氧化酶(SOD、CAT)活力和氧自由基(MDA、NO)含量相关免疫指标受影响越大,但对水解酶(ACP、AKP)活力无显著的影响,也表明斧足组织不是波纹巴非蛤代谢的主要场所,而是其体内抗氧化过程的关键场所。【意义】本研究结果为波纹巴非蛤空气暴露胁迫免疫生理指标的筛选和生理响应机制研究提供参考,并为波纹巴非蛤长途运输保鲜提供基础资料。[Background]Papia undulata is a famous economic shellfish in the South China Sea,mainly distributed in the coastal areas of Fujian,Guangdong,Guangxi and other provinces.Dry transport is the main method of the transportation of P.undulata.Long-term air exposure will affect the flavor of P.undulata and even lead to its death.[Objective]To investigate the effects of different air exposure time on immune related indices of P.undulata,a marine shellfish.[Methods]P.undulata was exposed to air for 0 h(control group),6 h,12 h and 24 h,respectively,and seawater culture was resumed after air exposure and the time nodes of seawater culture for 2,6,12 and 24 h,respectively,were restored.The indices of acid phosphatase(ACP),alkaline phosphatase(AKP),superoxide dismutase(SOD),catalase(CAT),malondialdehyde(MDA)and nitric oxide(NO)were determined after homogenization of the axilla unguiculata foot tissue at low temperature.[Results]Air exposure time within 24 h had no significant effect on the activities of hydrolase(ACP and AKP)(P>0.05),but had significant effects on the activities of antioxidant enzymes(SOD and CAT)and the content of oxygen free radicals(MDA and NO).Air exposure had a certain inhibitory effect on the activity of antioxidant enzymes,and the longer the air exposure time,the greater the fluctuation of SOD and CAT activities,and the more serious the oxidative damage.The longer the air exposure time,the higher the MDA content,the more serious the damage of P.undulata membrane,and the trend of NO content first increased and then decreased,which may be related to the protective effect of a large amount of NO produced by short time air exposure on the body.[Conclusion]The results indicate that the axe-foot tissue is not the main metabolic site of P.undulata,but the key site of antioxidant processes.The longer the air exposure time within 24 h,the greater the influence of antioxidant enzyme(SOD,CAT)activity and oxygen free radical(MDA,NO)content related immune indices,but had no significant effect on hydrolase(ACP,AKP)

关 键 词：波纹巴非蛤 空气暴露 斧足 免疫生理指标 酶活力 

分 类 号：S917.4[农业科学—水产科学]