circ_0001649抑制卵巢癌SKOV3细胞糖酵解、增殖、迁移及侵袭的机制研究  

circ_0001649 regulated glycolysis,proliferation,migration and invasion of ovarian cancer SKOV3 cells by targeting miR-223

作　　者：欧阳诗洁 熊琼英 李仙丽[1] OUYANG Shijie;XIONG Qiongying;LI Xianli(Xiaogan Hospital,Wuhan University of Science and Technology,Xiaogan 432000,Hubei,China)

机构地区：[1]武汉科技大学附属孝感医院,湖北孝感432000

出　　处：《西部医学》2024年第8期1143-1149,共7页Medical Journal of West China

摘　　要：目的探讨circ_0001649对卵巢癌SKOV3细胞糖酵解、增殖、迁移、侵袭的影响及其可能作用机制。方法选取2018年2月—2019年12月我院收治经病理诊断为卵巢癌的患者39例,采用qRT-PCR法检测卵巢癌组织与癌旁组织中circ_0001649、miR-223的表达水平;体外培养人卵巢癌细胞SKOV3,分别将pcDNA、pcDNA-circ_0001649、anti-miR-NC、anti-miR-223、pcDNA-circ_0001649与miR-NC、pcDNA-circ_0001649与miR-223 mimics转染至SKOV3细胞;观察SKOV3细胞中circ_0001649、miR-223的表达水平;检测葡萄糖消耗与乳酸、细胞增殖、迁移及侵袭能力。结果circ_0001649在卵巢癌组织表达水平低于癌旁组织表达,且miR-223的表达水平高于癌旁组织表达(P<0.05);转染pcDNA-circ_0001649或转染anti-miR-223可降低葡萄糖消耗及乳酸水平(P<0.05),明显降低OD值及Ki-67、MMP-2、MMP-9蛋白水平(P<0.05),减少迁移及侵袭细胞数(P<0.05);双荧光素酶报告实验证实circ_0001649可靶向结合miR-223;pcDNA-circ_0001649与miR-223 mimics共转染后可明显提高OD值及Ki-67、MMP-2、MMP-9蛋白水平(P<0.05),增加迁移及侵袭细胞数(P<0.05),提高葡萄糖消耗及乳酸水平(P<0.05)。结论circ_0001649过表达可对miR-223表达起到下调的作用,进而达到卵巢癌SKOV3细胞发生发展起到抑制作用。Objective To investigate the effect of circ_0001649 on glycolysis,proliferation,migration and invasion of ovarian cancer SKOV3 cells and its possible mechanism.Methods 39 cases of ovarin cancer patients diagnosed pathologically in our hospital from February 2018 to December 2019 were selected.The expression of circ_0001649,miR-224 in ovarian cancer tissues and adjacent tissues were detected by qRT PCR.In vitro culture of human ovarian cancer cell SKOV3,pcDNA and pcDNA circ_0001649,anti-miR-NC,anti-miR-223,pcDNA-circ_0001649 and miR-NC,pcDNA circ_0001649 and miR-223 mimics were transfected into SKOV3 cells.The circ in SKOV3 cells_0001649,miR-223 expression level were observed.Glucose consumption,lactic acid,cell proliferation,migration and invasion were detected.Results Compared with adjacent tissues,the expression level of circ_0001649 in ovarian cancer tissues was decreased(P<0.05),and the expression level of miR-223 was increased(P<0.05).Transfection of pcDNA-circ_0001649 or transfection of anti-miR-223 could significantly reduce glucose consumption and lactate level(P<0.05),and reduce OD value and the protein levels of Ki-67,MMP-2 and MMP-9(P<0.05),reduce the number of migration and invasion cells(P<0.05).The dual luciferase report experiment confirmed that circ_0001649 could target miR-223.Co-transfection of pcDNA-circ_0001649 and miR-223 mimics could significantly increase the OD value and the protein levels of Ki-67,MMP-2,and MMP-9(P<0.05),increase glucose consumption and lactate levels(P<0.05),and increase the number of migration and invasion cells(P<0.05).Conclusion circ0001649 overexpression can down regulate the expression of miR-223,thereby inhibiting the development of ovarian cancer SKOV3 cells.

关 键 词：circ_0001649 miR-223 卵巢癌 糖酵解 增殖 迁移 侵袭 

分 类 号：R737.31[医药卫生—肿瘤]