LncRNA FAS-AS1转染对K562细胞生物学行为的影响  

Effect of LncRNA Fas-AS1 transfection on biological behavior of K562 cells

作　　者：张峰华[1] 张彤如[1] 祝伟宏[1] 刘敬[1] 孙国文[1] ZHANG Fenghua;ZHANG Tongru;ZHU Weihong;LIU Jing;SUN Guowen(Chenzhou First People's Hospital,Chenzhou 423000,Hunan,China)

机构地区：[1]郴州市第一人民医院,湖南郴州423000

出　　处：《西部医学》2024年第8期1150-1155,共6页Medical Journal of West China

摘　　要：目的探究LncRNA FAS-AS1对K562白血病细胞生物学行为的影响。方法取对数生长期的K562细胞培养分为空白对照组(正常培养,不做任何处理),阴性对照组(转染LncRNA FAS-AS1空质粒),实验组(Lnc RNA FAS-AS1)。MTT法检测细胞增殖情况,Western blot检测细胞中mTOR、AMPK蛋白含量,流式细胞仪检测细胞凋亡情况。结果与空白对照组和阴性对照组相比,实验组K562细胞FAS-AS1 mRNA表达显著上升(P<0.05),但空白组与阴性对照组细胞中FAS-AS1 mRNA表达比较差异无统计学意义(P>0.05)。随着培养时间的延长,实验组K562细胞的增殖率显著低于对照组(P<0.05),但空白与阴性对照组细胞增殖率比较差异无统计学意义(P>0.05)。实验组凋亡率显著高空白对照和阴性对照组(P<0.05),而空白对照组与阴性对照组凋亡率比较差异无统计学意义(P>0.05)。实验组K562细胞AMPK蛋白表达水平显著高于空白对照组和阴性对照组(P<0.05),而空白对照组和阴性对照组K562细胞中表达水平蛋白mTOR、AMPK比较差异无统计学意义(P>0.05)。结论LncRNA FAS-AS1过表达对K562白血病细胞的生物学行为有显著影响,能够降低细胞的增殖数量及增殖活性,可能与活化AMPK基因进而阻断mTOR活性相关。Objective To explore the biological behavior of LncRNA FAS-AS1 on K562 leukemia cells.Methods The K562 cell culture in the logarithmic growth phase was divided into blank group(normal culture with no treatment),negative control group(transfected with LncRNA FAS-AS1 empty plasmid),and experimental group(LncRNA FAS-AS1).And the cell proliferation was detected by MTT method,the content of mTOR and AMPK proteins was detected by Western blot,and the difference of cell apoptosis was detected by flow cytometry.Results Compared with blank control group and negative control group,LncRNA Fas-AS1 content of K562 cells in experimental group was significantly increased(all P<0.05).However,there was no significant difference in mRNA content between blank and negative control cells(0.38±0.05 vs 0.39±0.06)(P>0.05).With the extension of culture time,the proliferation rate of K562 cells in experimental group(0.540±0.023)was significantly lower than that in control group(P<0.05),but there was no significant difference between blank and negative control group(0.851±0.040 vs 0.848±0.047)(P>0.05).The apoptosis rate of the experimental group was(25.13±5.22)%,significantly higher than that of the blank control group[(14.54±1.28)%]and the negative control group[(15.35±1.30)%](P<0.05).There was no significant difference between the blank control group and the negative control group(P>0.05).The expression of AMPK protein in K562 cells in the experimental group was significantly higher than that in the control group(P<0.05),while there was no significant difference in the protein content in K562 cells between the two groups(P>0.05).Conclusion LncRNA Fas-AS1 overexpression can significantly affect the biological behavior of K562 leukemia cells,and can reduce the number and proliferation activity of cells,which may be related to the activation of AMPK gene and the blocking of mTOR activity.

关 键 词：LncRNA FAS-AS1 白血病 MTOR AMPK 细胞生物行为 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R733.7[医药卫生—基础医学]