二氢槲皮素和β-烟酰胺单核苷酸顺势和逆势给药对健康大鼠生长和生理的影响  

Effects of homeopathic and counter current administration of dihydroquercetin andβ-nicotinamide mononucleotide on growth and physiology in Rats

作　　者：甘永金[1] 梁丹玉[1] 陈瑞泽 蓝保强 蒋盛军 GAN Yong-Jin;LIANG Dan-Yu;CHEN Rui-Ze(Institute of occupational health and toxicity evaluation,Guangxi Zhuang Autonomous Region Research Academy of occupational disease prevention and control,Nanning 530021,Guangxi,China)

机构地区：[1]广西壮族自治区职业病防治研究院职业卫生与毒性评价所,广西南宁530021 [2]广西壮族自治区中医药研究院制剂中心 [3]广西壮族自治区中药质量标准研究重点实验室

出　　处：《吉林医学》2024年第8期1783-1788,共6页Jilin Medical Journal

基　　金：广西壮族自治区中医药研究院自主研究项目[项目编号:桂中自研2021004]。

摘　　要：目的:通过研究二氢槲皮素和β-烟酰胺单核苷酸顺势和逆势给药对健康大鼠生长和生理的影响,进一步验证中医阴阳分药分时服用方剂学理论。方法:48只无特定病原体级SD大鼠,随机分为顺势饲喂组、逆势饲喂组、混合饲喂组、对照组各12只,雌雄各半,顺势饲喂组早晨给予二氢槲皮素溶液,下午给予β-烟酰胺单核苷酸溶液,逆势饲喂组给药顺序与顺势饲喂组相反,混合饲喂组给予二氢槲皮素和β-烟酰胺单核苷酸混合溶液灌胃,分早晚两次给药,对照组早晚给予灌胃等量超纯水,四组均每周给药5 d,共给药6周。分别于饲喂第1天、第10天、第20天、第30天、第45天时观察各组大鼠的皮肤毛发、行为状态、兴奋程度、激怒反应、睡眠状态、大便状态。分别于饲喂后第2周、第4周、第6周的周一、周三、周五测定大鼠采食量和体重,饲喂第45天检测大鼠的血液常规和血生化指标,计算脏器系数。结果:实验期间,各组大鼠的皮肤毛发、行为状态、兴奋程度、激怒反应、睡眠状态、大便状态均未见明显改变。四组雌、雄性大鼠在各个阶段的日均采食量和体重比较差异均无统计学意义(P>0.05)。给药6周后,四组大鼠肝、脾、肺、肾、胃脏器系数、白细胞计数、中性粒细胞计数、淋巴细胞计数、单核细胞计数、嗜酸性粒细胞计数、红细胞计数、血红蛋白、血清总蛋白、总胆红素、碱性磷酸酶、三酰甘油、血清总胆固醇、尿素、空腹血糖、肌酐、钾离子比较,差异均无统计学意义(P>0.05)。雄性大鼠顺势饲喂组和逆势饲喂组心脏脏器系数低于对照组(P<0.05);雌性大鼠顺势饲喂组谷丙转氨酶高于对照组(P<0.05);雄性大鼠混合饲喂组谷草转氨酶高于对照组(P<0.05);雌性大鼠混合饲喂组钠离子低于对照组,差异均有统计学意义(P<0.05)。结论:顺势给药和逆势给药可引起大鼠心脏脏器系数降低,混Objective In order to further validate the traditional Chinese medicine formula-ology theory of yin-yang medicine division and time-division by studying the effects of administering dihydroquercetin ANDβ-nicotinamide mononucleotides in both homeopathic and retrograde ways on the growth and physiology of rats.Method 48 SD rats without specific pathogen level were randomly divided into homeopathic feeding group,counter feeding group,mixed feeding group,and control group,with 12 rats in each group,half male and half female.The homeopathic feeding group was given dihydroquercetin solution in the morning and in the afternoonβ-Nicotinamide mononucleotide solution was administered in the reverse order to the homeopathic feeding group,while the mixed feeding group received dihydroquercetin andβ-Niacinamide mononucleotide mixed solution was administered by gavage twice in the morning and evening.The control group was given an equal amount of ultrapure water by gavage in the morning and evening.All four groups were administered 5 days a week for a total of 6 weeks.On the 1st,10th,20th,30th,and 45th day of feeding,observe the skin hair,behavioral status,excitement level,irritability response,sleep status,and fecal status of rats in each group.The feed intake and body weight of rats were measured on Mondays,Wednesdays,and Fridays after 2,4,and 6 weeks of feeding.On the 45th day of feeding,blood routine and biochemical indicators of rats were measured,and organ coefficients were calculated.Results During the experiment,there were no significant changes in the skin hair,behavioral state,excitement level,irritability response,sleep state,and fecal state of the rats in each group.There was no statistically significant difference in daily feed intake and body weight between the four groups of female and male rats at each stage(P>0.05).After 6 weeks of administration,there were no statistically significant differences in organ coefficients,white blood cell count,neutrophil count,lymphocyte count,monocyte count,eosinophil count,

关 键 词：阴阳五行 方剂学 二氢槲皮素 β-烟酰胺单核苷酸 顺势给药 逆势给药 

分 类 号：R33[医药卫生—人体生理学]