茶树炭疽病生防菌的筛选、鉴定及拮抗机制研究  

Screening,identification and antagonistic mechanism for biological control of tea anthracnose

作　　者：李红莉[1] 卢健 赵芸[1] 黄海涛[1] 崔宏春[1] 朱建杰[2] 任国华[2] LI Hongli;LU Jian;ZHAO Yun;HUANG Haitao;CUI Hongchun;ZHU Jianjie;REN Guohua(Hangzhou Academy of Agricultural Sciences,Hangzhou 310024,Zhejiang;Agricultural Technology Promotion Center of Yuhang District of Hangzhou City,Hangzhou 311121,Zhejiang)

机构地区：[1]杭州市农业科学研究院,浙江杭州310024 [2]杭州市余杭区农业技术推广中心,浙江杭州311121

出　　处：《浙江农业科学》2024年第8期1902-1907,共6页Journal of Zhejiang Agricultural Sciences

基　　金：杭州市农业科技协作与创新攻关项目(杭农〔2021〕58号);浙江省(茶树)农业新品种选育重大科技专项(2021C02067-4);杭州市农业科学研究院创新基金项目(2022HNCT-04);国家茶叶产业技术体系建设专项(CARS-23)。

摘　　要：为了获得对茶树炭疽菌具有较好拮抗效果的生防菌株,从茶树根际土壤中采用梯度稀释法分离纯化得到细菌63株,通过平板对峙法筛选出1株对茶树炭疽菌具有稳定拮抗作用的菌株HZ-01,其对茶树炭疽菌菌丝生长抑制率达到71.5%。结合菌株形态学、生理生化特征及16S rDNA序列比对分析,将菌株HZ-01鉴定为贝莱斯芽孢杆菌(Bacillus velezensis)。胞外水解酶活性分析显示,HZ-01菌株具有产生β-1,3-葡聚糖酶、蛋白酶和纤维素酶的能力。通过显微镜观察发现,HZ-01的发酵产物对茶树炭疽菌的菌丝生长和孢子萌发具有明显的抑制作用。以上结果表明,菌株HZ-01在防控茶树炭疽病方面具有很好的研究价值和应用潜力。In order to obtain antagonistic bacteria for the biocontrol of tea anthracnose,63 strains of bacteria were isolated and purified from the rhizosphere soil of tea plants by dilution plate method.Among these isolates,strain HZ-01 showed the highest inhibition rate(71.5%).Combined with morphological characteristics,physiological and biochemical characteristics and 16S rDNA sequence comparison analysis,strain HZ-01 was identified as Bacillus velezensis.Extracellular hydrolase activity analysis showed that strain HZ-01 could produceβ-1,3-glucanase,protease and cellulase.The fermentation products had an obvious inhibitory effect on the mycelial growth and spore germination of Colletotrichum camelliae.The above results indicated that strain HZ-01 could be used as a candidate strain for biological control of tea anthracnose.

关 键 词：茶树炭疽菌 芽孢杆菌 分离鉴定 生物防治 

分 类 号：S435.71[农业科学—农业昆虫与害虫防治]