MOFs孔结构对固定化酶性能影响的研究进展  

Research progress in effect of MOFs pore structure on properties of immobilized enzymes

作　　者：程凯鹏 张申梦 李立鑫 魏梓悦 黄霞辉 赵俭波[2] CHENG Kaipeng;ZHANG Shenmeng;LI Lixin;WEI Ziyue;HUANG Xiahui;ZHAO Jianbo(School of Chemistry and Chemical Engineering,Zhoukou Normal University,Zhoukou 466000,Henan,China;School of Chemistry and Chemical Engineering,Tarim University,Alar 843300,Xinjiang,China)

机构地区：[1]周口师范学院化学化工学院,河南周口466000 [2]塔里木大学化学化工学院,新疆阿拉尔843300

出　　处：《精细化工》2024年第8期1647-1657,共11页Fine Chemicals

基　　金：国家自然科学基金项目(22268038);河南省重点研发与推广专项(科技攻关)项目(222102310430);周口师范学院高层次人才科研启动经费资助项目(ZKNUC2021017)。

摘　　要：固定化酶是实现酶制剂产业化应用的有效途径,载体是影响固定化酶催化效果的关键因素。金属有机框架(MOFs)是结构高度有序的多孔材料,具有构效关系明确、孔结构均一、孔道性质与化学性质在原子层面可调、可设计和易化学修饰等特点,近年来,其作为酶载体方面的研究已有较大的进展。该文以MOFs孔结构对固定化酶性能的影响为主题,分析了MOFs孔结构的影响因素,总结了酶在笼型空穴与通道型孔这2种典型孔中负载的研究进展,详细论述了MOFs孔结构对酶的固定化、活性等方面的影响。最后,指出多级孔在固定化酶强化传质方面的特殊作用以及开发孔-酶匹配MOFs的重要性,展望了利用MOFs自身催化能力在纳米尺度内构建高效化学-酶级联催化剂的发展优势,以及通过调控微环境强化酶催化过程的可行性。Enzyme immobilization is an effective approach to promote its industrial applications,with the carrier the key influencing factor on the catalytic performance of immobilized enzymes.Metal-organic frameworks(MOFs),highly ordered porous materials with clear structure-function relationships,uniform pore size distribution,tunable and designable porosity at the atomic level,have made significant progress as enzyme carrier.This review focused on the influence of MOFs pore structure on their enzyme immobilization performance.Herein,the influencing factors on MOFs pore structure were analyzed,followed by research progress summarization on enzyme loading in cage-type cavities and channel-type pores with a detailed description on the influence of porosity on enzyme immobilization,enzyme activity etc.Finally,the special role of MOFs hierarchical-structure in enhancing mass transfer with immobilized enzymes and the importance of developing pore-enzyme matching MOFs were pointed out,while the development advantages of utilizing MOFs autocatalytic ability to construct high-efficiency chemical-enzyme cascade catalysts in nanometer scale and the feasibility of enhancing enzyme catalytic process by regulating microenvironment were discussed.

关 键 词：固定化酶 金属有机框架 孔结构 笼型空穴 通道型孔 

分 类 号：Q814[生物学—生物工程]