TCF21在肺腺癌中的表达及其对肿瘤血管生成的影响  

Expression of TCF21 in lung cancer and its effect on angiogenesis

作　　者：娄宁 吴婷婷 赵彤 吕花营 LOU Ning;WU Ting-ting;ZHAO Tong;LV Hua-ying(Department of Pathology,the Eighth People's Hospital of Ji'nan City,Ji'nan 271104,China;Department of Chinese Medicine,the Eighth People's Hospital of Ji'nan City,Ji'nan 271104,China)

机构地区：[1]济南市第八人民医院病理教研室,济南271104 [2]济南市第八人民医院中医科,济南271104

出　　处：《诊断病理学杂志》2024年第7期624-628,共5页Chinese Journal of Diagnostic Pathology

基　　金：山东省医药卫生科技发展计划项目(编号:02201040299);济南市卫生健康委员会科技发展计划(编号:2022-2-88;2023-2-94)。

摘　　要：目的探讨核转录因子21(TCF21)在肺腺癌组织中的表达及其对肺腺癌血管生成的影响。方法收集济南市第八人民医院配对新鲜肺腺癌组织标本20例,采用蛋白质免疫印迹(Western blot)方法检测TCF21在肺腺癌组织和癌旁组织中的表达。选用人肺腺癌细胞系A549,通过慢病毒感染肺腺癌细胞,构建TCF21过表达稳转细胞株,利用Western blot技术检测对照组、空载组和过表达组缺氧诱导因子-1α(HIF-1α)、血管内皮生长因子A(VEGFA)蛋白表达量,并通过体外血管形成实验观察TCF21对人脐静脉内皮细胞(HUVEC)成管能力的影响。结果TCF21在新鲜肺腺癌组织中的表达低于对应的癌旁组织(P<0.05)。肺腺癌A549细胞系过表达TCF21后,与对照组MOCK组、对照组Vector组相比,HIF-1α、VEGF蛋白表达显著降低,成管实验显示HUVEC的管长度和节点明显减少。结论TCF21在肺腺癌组织中低表达,具有抑制肺腺癌血管生成能力,为临床抗肿瘤治疗提供新的潜在靶点。Objective To investigate the expression of nuclear transcription factor 21(TCF21)in lung adenocarcinoma and its effect on the angiogenesis.Methods A total of 20 matched fresh lung adenocarcinoma tissue specimens werecollected from the Eighth People's Hospital of Jinan City.Western blot was used to detect the expressionof TCF21 in lung adenocarcinoma and adjacent tissues.Human lung cancer cell line A549 was infected with lentivirus,and TCF21overexpression stable cell line was constructed,Western blot was used to detect the protein expression levels ofhypoxia-inducing factor-1α(HIF-1α)and vascular endothelial growth factor(VEGF)in the control group,no-load groupand overexpression group.The effect of TCF21 on the tube-forming ability of human umbilical vein endothelial cells(HUVEC)was observed by in vitro angiogenesis experiment.Results The expression of TCF21 in fresh lung adenocarcinoma tissue was lower than that in adjacent tissues(P<0.05).After overexpression of TCF21 in lungadenocarcinoma A549 cell line,the protein expression of HIF-1αand VEGF was significantly decreased compared withthe MOCK group and Vector group in the control,and the tube length and nodes of HUVEC were significantly reduced in tube formation experiments.Conclusion TCF21 is lowly expressed in lung adenocarcinoma tissue,and has theability to inhibit the angiogenesis of lung adenocarcinoma,providing a new potential target for clinical anti-tumor therapy.

关 键 词：肺肿瘤 TCF21 血管生成 血管内皮生长因子 缺氧诱导因子-1Α 

分 类 号：R734.2[医药卫生—肿瘤]