运输水温对曼氏无针乌贼成体死亡率及生理指标的影响  

Effects of water temperature in transportation on mortality and physiological indicators of Sepiella japonica

作　　者：蒋永生 周珊珊 周永东[1,2] 徐开达 镇小蔓[1] 焦礼诗 张秋宏[1] 渠遥 JIANG Yongsheng;ZHOU Shanshan;ZHOU Yongdong;XU Kaida;ZHEN Xiaoman;JIAO Lishi;ZHANG Qiuhong;QU Yao(Marine and Fisheries Research Institute,Zhejiang Ocean University,Zhoushan 316021,China;Zhejiang Marine Fisheries Research Institute/Key Research Station for Fisheries Resources of Main Fishing Ground,Ministry of Agriculture and Rural Affairs/Key Research Station of Sustainable Utilization for Marine Fisheries Resources,Zhoushan 316021,China)

机构地区：[1]浙江海洋大学海洋与渔业研究所,浙江舟山316021 [2]浙江省海洋水产研究所/农业农村部重点渔场渔业资源科学观测实验站/浙江省海洋渔业资源可持续利用技术研究重点实验室,浙江舟山316021

出　　处：《南方水产科学》2024年第4期107-115,共9页South China Fisheries Science

基　　金：国家重点研发计划“蓝色粮仓科技创新”重大专项(2020YFD09008004,2019YFD0901204);国家重点研发计划项目(2023YFD2401900);浙江省重点研发计划项目(2019C02056)。

摘　　要：增殖放流是曼氏无针乌贼(Sepiella japonica)渔业资源修复的重要手段,而水温可能会影响增殖放流运输过程中乌贼的生存状态。为优化曼氏无针乌贼成体增殖放流运输技术,通过实验室内模拟不同水温(14、17、20、23、26、29℃)下的运输过程,分析了不同运输温度下乌贼死亡率和肝脏生理指标的变化。结果显示,运输水温显著影响乌贼成体死亡率(p<0.05),14和29℃处理组的死亡率较高,分别为30.0%和63.3%。相较于20℃处理组,肝糖原含量在14和17℃下显著下降,在29℃下显著上升(p<0.05);乳酸含量在23和29℃下显著上升(p<0.05);超氧化物歧化酶(SOD)活性在17℃下显著下降,在26℃下显著上升(p<0.05);碱性磷酸酶(AKP)活性在各水温下显著下降(p<0.05);随水温变化,谷草转氨酶(AST)活性先下降后上升,而谷丙转氨酶(ALT)活性则先上升后下降。乌贼死亡率与肝脏糖原、乳酸含量和AST活性显著正相关(p<0.05)。研究表明,在5只·L^(-1)密度下水温过低或过高均不适宜乌贼运输,建议水温控制在17~26℃。Restocking and releasing is an important means to restore the resources of Sepiella japonica,and the water temperature during the transportation process of restocking and releasing may affect its survival status.In order to optimize the transportation techniques for the adult enrichment and release of S.japonica,we conducted an experiment to simulate the transportation environment in laboratory and performed transportation experiments of adult S.japonica with different water temperatures(14,17,20,23,26,29℃),and explored its mortality rate and liver physiological indicators.The results show that the water temperature in transportation affected the mortality rate significantly(p<0.05),and the mortality rates in 14 and 29℃ groups were higher,reaching 30.0% and 63.3%,respectively.Compared with 20℃ group,the glycogen content decreased significantly in 14 and 17℃groups,but increased significantly in 29℃ group(p<0.05).The lactic acid content increased significantly in 23 and 29℃ groups(p<0.05).SOD decreased significantly in 17℃ group but increased significantly in 26℃ group(p<0.05).AKP decreased significantly at all water temperatures(p<0.05).With the change of water temperature,AST decreased initially and then increased,while ALT increased initially and then decreased.The mortality rate of squids was significantly positively correlated with glycogen,lactic acid content and AST activity in liver(p<0.05).In conclusion,too low or too high water temperature is not suitable for squid transportation at a density of 5 ind·L−1,and it is recommended to control the water temperature between 17℃ and 26℃.

关 键 词：曼氏无针乌贼 模拟运输 水温 死亡率 生理指标 

分 类 号：S981.3[农业科学—捕捞与储运]