全反式维甲酸诱导综合征型唇腭裂小鼠模型的建立和分析  

Establishment and Analysis of A Mouse Model of Syndromic Cleft Lip and Palate Induced by All-trans Retinoic Acid.

作　　者：王宇萌 迟丹丹 张贝贝 段小红 黄永清[1,3] WANG Yumeng;CHI Dandan;ZHANG Beibei;DUAN Xiaohong;HUANG Yongqing(Ningxia Medical University,Yinchuan 750004,China;State Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration,National Clinical Research Center for Oral Disease,Shaanxi Key Laboratory of Stomatology,Department of Oral Biology&Clinic of Oral Rare Diseases and Genetic Diseases,School of Stomatology,the Fourth Military Medical University,Xi’an 710032,China;Ningxia Key Laboratory of Oral Disease Research,Ningxia Key Laboratory of Craniomaxillofacial Deformities Research,Department of Oral and Maxillafacial Surgery,Hospital of Stomatology,the General Hospital of Ningxia Medical University,Yinchuan 750004,China.)

机构地区：[1]宁夏医科大学口腔医学院,宁夏银川750004 [2]口颌系统重建与再生全国重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔医学重点实验室,空军军医大学第三附属医院口腔生物学教研室,陕西西安710032 [3]宁夏医科大学总医院口腔医院口腔颌面外科,宁夏口腔疾病研究重点实验室,宁夏银川750004

出　　处：《口腔医学研究》2024年第8期710-714,共5页Journal of Oral Science Research

基　　金：国家自然科学基金(编号:81960197、81974145、81771052);陕西省重点研发计划重点产业创新链(群)重点项目(编号:2021ZDLSF02-13);国家口腔疾病临床研究中心重点项目(编号:LCC202201);宁夏医科大学总医院(自治区临床研究中心)开放课题(编号:2019209)。

摘　　要：目的:建立综合征型唇腭裂小鼠模型并分析其表型特征。方法:采用灌胃法于孕鼠妊娠10.5 d(gestational days 10.5,GD10.5),以90 mg/kg的全反式维甲酸(all-trans retinoic acid,atRA)或等量玉米油+二甲基亚砜(dimethyl sulfoxide,DMSO)溶剂处理,GD14.5/GD17.5取胎鼠,行体视显微镜、石蜡包埋、切片、苏木精-伊红(hematoxylin-eosin,HE)染色观察胎鼠的全身及腭组织结构和发育情况;免疫荧光染色验证维甲酸受体(retinoic acid receptor alpha,RARα)在小鼠腭部及牙的表达情况。结果:实验组胎鼠出现腭裂、磨牙发育异常、肢体畸形、心肌致密化不全等表型。实验组RARα表达升高。结论:atRA诱导建立的小鼠腭裂模型具有综合征型唇腭裂的特征,可通过激活RARα受体影响小鼠发育。Objective:To establish and analyze a mouse model of syndromic cleft lip and palate in mice.Methods:Pregnant mice on gestation day 10.5(GD10.5)were gavaged with 90 mg/kg of atRA or corn oil and DMSO solvent.Upon euthanasia of pregnant mice on GD17.5,embryos were collected.Stereomicroscope,paraffin embedding and sectioning,and hematoxylin-eosin(HE)staining were used to observe the development of embryos.Immunofluorescence staining was used to verify the expression of retinoic acid receptor alpha(RARα).Results:The embryos in the atRA group showed cleft palate,limb deformities,abnormal molar development,and non-compaction of ventricular myocardium.Immunofluorescence staining showed the expression of RARαincreased in molar and palate of embryos of the atRA group.Conclusion:The mouse cleft palate model induced by atRA presents as syndromic cleft lip and palate,and atRA affects the development of mice by activating of RARαreceptor.

关 键 词：全反式维甲酸 综合征型唇腭裂 先天性心脏病 磨牙 成釉细胞 

分 类 号：R-332[医药卫生] R782.2