2012⁃2023年度滨州市甲型H3N2流感病毒基因进化特征分析  

Phylogenetic Analysis of Influenza A/H3N2 in Binzhou,China During 2012-2023

作　　者：张丽芳[1] 尹秀升 赵娜娜 张静[1] 黄莹[1] 张美英[1] ZHANG Lifang;YIN Xiusheng;ZHAO Nana;ZHANG Jing;HUANG Ying;ZHANG Meiying(Binzhou Center for Disease Control and Prevention,Binzhou 256600,China)

机构地区：[1]滨州市疾病预防控制中心,滨州256600

出　　处：《病毒学报》2024年第4期830-836,共7页Chinese Journal of Virology

摘　　要：为分析滨州市2012-2023年流感季节甲型H3N2流感的遗传和抗原特征,本文采集滨州市2012至2023年的流感监测哨点医院流感样病例咽拭子标本进行病毒分离,选取45株甲型H3N2流感病毒代表毒株进行全基因组序列测定,通过生物信息学软件MegAlign,MEGA分析基因进化特征。2012-2023年度监测标本10137份,季节性流感(甲型H3N2、甲型H1N1、BY系、BV系)病毒总阳性率16.2%,8个年度监测到甲型H3N2流感病毒,阳性率分别为9.6%、1.6%、6.0%、0.1%、10.4%、7.3%、19.0%、8.8%,差异有统计学意义(χ2=775.902,P<0.001)。45株病毒的全基因组8个片段序列相似性中位数97.2%~99.0%。各片段均位于相应分支,未发现基因重配。HA基因上128(A-T-A-T),131(T-K-T-K),135(T-K-T),138(A-S-A)位点有回复突变现象。2012-2023年度滨州市甲型H3N2流感病毒全基因组序列监测到3次演化,位于4个主要分支,引起分支演化的HA基因的突变位点位于抗原决定簇,抗原漂移是引起分支演化的基础。To analyze the genetic and antigenic characterization of influenza A/H3N2 virus in Binzhou,China during 2012-2023.Throat swabs were collected from influenza-like illness(ILI)from 2012 to 2023 in Binzhou,China.Virus isolation was performed.45 representative influenza A/H3N2 isolates were selected for whole genome sequencing.Phylogenetic analysis of whole-genome sequences was performed using MegAlign and MEGA software.A total of 10137 samples were obtained and the overall positive rate of influenza virus(A/H3N2,A/H1N1,BY、BV)was 16.2%.Our surveillance data indicated that Influenza A/H3N2 virus was detected in eight years,the positive rates were 9.6%,1.6%,6.0%,0.1%,10.4%,7.3%,19.0%and 8.8%,respectively.Statistically significant differences between the positive rate of influenza A/H3N2 viruses were observed(χ2=775.902,P<0.001).The median pairwise sequence identities were ranging from 97.2%~99.0%for 8 segments of 45 virus sequences.Segment of all A/H3N2 viruses analyzed belonged to the corresponding subclade,reassortment was not observed.There were revert mutations at 128(A-T-A-T),131(T-K-T-K),135(T-K-T),138(A-S-A)sites on HA gene.The whole-genome sequence of influenza A/H3N2 virus detected three evolvement,which were located in four main genetic clades during 2012-2023.The mutation sites were located at antigenic determinant positions of HA gene which caused the genetic clade evolution.Antigenic drift variant is the basis of genetic clade evolution of influenza viruses.

关 键 词：甲型H3N2流感病毒 全基因组序列 同源性 分支演化 

分 类 号：R373.1[医药卫生—病原生物学]