玉竹地上部位化学成分及其α-葡萄糖苷酶抑制活性研究  

Study on the Chemical Constituents from the Aerial Parts of Polygonatum odoratum andα-Glucosidase Inhibitory Activity

作　　者：安涑闽 王亚凤[2] 葛利 何瑞杰[2] 庞闹 黄永林[2] 杨克迪 AN Su-min;WANG Ya-feng;GE Li;HE Rui-jie;PANG Nao;HUANG Yong-lin;YANG Ke-di(Medical College of Guangxi University,Nanning 530004,China;Guangxi Institute of Botany,Chinese Academy of Science,Guangxi Zhuang Autonomous Region/Guangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization,Guilin 541006,China)

机构地区：[1]广西大学医学院,广西南宁530004 [2]广西壮族自治区中国科学院广西植物研究所/广西植物功能物质与资源持续利用重点实验室,广西桂林541006

出　　处：《中药材》2024年第2期365-370,共6页Journal of Chinese Medicinal Materials

基　　金：广西科技重大专项(桂科AA22096020);广西重点研发计划(桂科AB22035019);桂林市创新平台与人才计划(20210102-3);国家自然科学基金项目(82060764,32060108)。

摘　　要：目的:研究玉竹地上部位的化学成分及其对α-葡萄糖苷酶的抑制活性。方法:采用大孔吸附树脂HP-20、Sephadex LH-20、MCI gel CHP 20P、Chromatorex C18、半制备型高效液相等色谱法对玉竹地上部位75%甲醇提取物进行分离纯化,通过1H-NMR、13C-NMR等波谱技术对化合物进行结构鉴定,并采用α-葡萄糖苷酶活性筛选模型对部分化合物进行活性筛选。结果:从玉竹地上部位中分离得到16个化合物,分别鉴定为:对羟基苯甲酸(1)、芹菜素-6-C-葡萄糖苷(2)、异金雀花素(3)、异槲皮苷(4)、肥皂草苷(5)、山柰酚-3-O-β-芸香苷(6)、芦丁(7)、异鼠李素-3-O-芸香苷(8)、牡荆素-2″-O-葡萄糖苷(9)、牡荆素-4″-O-葡萄糖苷(10)、牡荆素-2″-O-β-D-(6‴-乙酰基)-葡萄糖苷(11)、异牡荆素-2″-O-β-D-葡萄糖苷(12)、芹菜素-6-C-阿拉伯糖-葡萄糖苷(13)、槲皮素-7-O-[α-L-鼠李糖-(1→6)-β-D-半乳糖苷](14)、金圣草黄素-7-O-槐糖苷(15)、金圣草黄素-7-O-[β-D-芹菜糖-(1→6)]-β-D-葡萄糖苷(16),并筛选了部分化合物的α-葡萄糖苷酶抑制活性。结论:其中,化合物2~6、10~16为首次从该植物中分离得到。化合物2具有显著的α-葡萄糖苷酶抑制活性。Objective:To study the chemical constituents from the aerial parts of Polygonatum odoratum and the inhibition onα-glucosidase activity.Methods:The 75%methanol extract from the aerial parts of Polygonatum odoratum was isolated and purified by various chromatographic techniques,such as macroporous resin HP-20,Sephadex LH-20,MCI gel CHP 20P,Chromatorex C18,semi-preparative HPLC and others.The structures were identified on the basis of 1H-NMR,13C-NMR.In addition,theα-glucosidase inhibitory activity of the compounds were evaluated by pharmacological methods simultaneously.Results:Sixteen compounds were isolated from the aerial parts of Polygonatum odoratum and identified as p-hydroxybenzoic acid(1),6-C-glucosylapigenin(2),isoscoparine(3),isoquercitrin(4),saponarin(5),kaempferol-3-O-β-rutinoside(6),rutin(7),isorhamnetin-3-O-rutinoside(8),2″-O-glucosylvitexin(9),4″-Oglucosylvitexin(10),vitexin-2″-O-β-D-(6‴-acetyl)-glucoside(11),isovitexin-2″-O-β-D-glucoside(12),apigenin-6-C-arabinosylglucoside(13),quercetin-7-O-[α-L-rhamnopyranosyl(1→6)-β-D-galactopyranoside](14),chrysoeriol-7-O-sophoroside(15),7-O-[β-Dglicopiranosil-(1→6)-β-D-apiofuranosil]crisoeriol(16),respectively.Some of the compounds were also screened forα-glucosidase inhibitory activity.Conclusion:Among them,compounds 2 to 6,10 to 16 are isolated from Polygonatum odoratum for the first time,compound 2 shows significant inhibitory effect onα-glucosidase.

关 键 词：玉竹 化学成分 Α-葡萄糖苷酶 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学] R285.5