川西獐牙菜抑菌活性部位的制备及其谱效关系的分析  

Preparation of antibacterial active parts of Swertia mussoti and comparison of spectrum-effect relationship

作　　者：孙兆泉 乔茂旺 李彩云 陈海娟 尚军 SUN Zhaoquan;QIAO Maowang;LI Caiyun;CHEN Haijuan;SHANG Jun(Qinghai Province Key Laboratory of Medicinal Animal and Plant Resources on the Qinghai-Tibet Plateau,School of Life Sciences,Qinghai Normal University,Xining,Qinghai,810000 P.R.China;Institute of Plateau Science and Sustainable Development,Xining,Qinghai,810000P.R.China)

机构地区：[1]青海师范大学生命科学学院,青海省青藏高原药用动植物资源重点实验室,青海西宁810000 [2]高原科学与可持续发展研究院,青海西宁810000

出　　处：《华西药学杂志》2024年第4期387-391,共5页West China Journal of Pharmaceutical Sciences

基　　金：青海省科技厅国际合作项目(2021-HZ-805);青海省“昆仑英才·高端创新创业人才”项目(2020)。

摘　　要：目的制备川西獐牙菜的抑菌活性部位并建立其HPLC谱效关系指纹图谱。方法采用三相萃取法制备川西獐牙菜的活性部位,体系1用乙腈-二氯甲烷-石油醚-水(5:1:5:5)、体系2用乙腈-乙酸乙酯-石油醚-水(1:1:2:1)萃取;通过牛津杯法测定各部位的抑菌活性,采用Pearson相关性分析与正交偏最小二乘判别分析研究HPLC指纹图谱的谱效关系。结果川西獐牙菜抑菌活性成分主要富集于体系1、2的中相,6个不同部位共有峰20个,其中,7、12、15、16、19、20号峰与抑菌活性呈显著正相关。结论所用方法可有效富集川西獐牙菜的活性成分,可为川西獐牙菜抑菌活性成分的制备及质量控制与评价提供参考。OBJECTIVE To prepare the bacteriostatic active site of Swertia mussoti Franch and construct the HPLC fingerprint based on three-phase extraction.METHODS The active parts of S.mussoti were prepared by system 1(acetonitrile-dichloromethane-petroleum ether-water=5:1:5:5:5)and system 2(acetonitrile-ethyl acetate-petroleum ether-water=1:1:2:1),the antimicrobial activity of each part was determined,and the spectral-effect relationship was investigated by Pearson correlation analysis and OPLS-DA.RESULTS The antibacterial activity of S.mussoti was accumlated in the middle phase of system 1 and system 2.A total of 20 same peaks were identified in 6 different parts,with No.7,No.12,No.15,No.16,No.19 and No.20 peaks exhibiting a significant positive correlation with the antibacterial activity.CONCLUSION This method can effectively enrich the active ingredients of S.mussoti,and provide a reference basis for the preparation,quality control and evaluation of the antibacterial active ingredients of S.mussoti.

关 键 词：川西獐牙菜 三相萃取 抑菌活性 谱效关系 指纹图谱 相关性分析 活性筛选 质量控制 

分 类 号：R96[医药卫生—药理学]