核桃粕酶解物抑制脂质积累的机制  

The Mechanism of Walnut Meal Enzymatic Hydrolysate Inhibiting Lipid Accumulation

作　　者：白玉英 解静 彭麟杰 毛家英 李世俊[1] 田洋[2,3] Bai Yuying;Xie Jing;Peng Linjie;Mao Jiaying;Li Shijun;Tian Yang(College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201;National Research and Development Professional Center for Moringa Processing Technology,Yunnan Agricultural University,Kunming 650201;Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources,Ministry of Education,Yunnan Agricultural University,Kunming 650201)

机构地区：[1]云南农业大学食品科学技术学院,昆明650201 [2]云南农业大学云南省生物大数据重点实验室,昆明650201 [3]云南农业大学食药同源资源开发与利用教育部工程中心,昆明650201

出　　处：《中国食品学报》2024年第7期193-200,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：云南省科技厅重大科技专项计划项目(202002AA100005,202102AE090027-2);云南省科技厅云南绿色食品国际合作研究中心项目(2019ZG00905)。

摘　　要：利用分化的3T3-L1脂肪细胞和高脂饮食(HFD)诱导的肥胖C57BL/6小鼠模型,研究核桃粕酶解物的抗肥胖作用。采用油红O脂肪染色法检测脂肪细胞脂质积累情况,采用试剂盒检测细胞甘油三脂(TG)、甘油(Gly)和游离脂肪酸(FFA)含量,采用实时荧光定量PCR(PCR RT-qPCR)检测脂肪合成相关基因(mRNA)的表达水平。结果表明,核桃粕酶解物显著降低脂肪细胞内脂滴聚积,显著降低脂肪细胞内TG和FFA的含量,增加细胞上清液中Gly含量,同时核桃粕酶解物显著降低脂肪细胞中脂肪合成相关基因PPAR-γ和C/EBPαmRNA表达水平;当核桃粕酶解物的质量浓度达到50μg/mL时,TG和FFA分别降低了40.14%和39.50%,Gly水平增加了35.12%;PPAR-γ和C/EBPα的mRNA表达水平分别降低了31.42%和55.25%。此外,核桃粕酶解物显著降低HFD小鼠的体质量、脂肪组织质量和食物摄入量。研究表明,核桃粕酶解物可以抑制3T3-L1脂肪细胞脂质积累,其作用机制与抑制脂质合成相关基因表达相关。此外,其还具有抑制HFD脂质积累的作用。The anti-obesity effects of walnut meal enzymatic digests were investigated using a C57BL/6 mouse model of high-fat diet(HFD)-induced obesity and a differentiated 3T3-L1 adipocyte model.The accumulation of lipids in the adipocytes was identified through the use of oil red O fat staining.The levels of triglycerides(TG),glycerol(Gly),and free fatty acids(FFA)within the cells were quantified using a commercially available kit.The expression of genes involved in fat synthesis was determined through real-time fluorescence quantitative polymerase chain reaction(PCR RT-qPCR).The results demonstrated that the enzymatic digestion of walnut meal markedly diminished the accumulation of lipid droplets in adipocytes,notably reduced the TG and FFA content in adipocytes,and enhanced the Gly content in the cell supernatant.Concurrently,the enzymatic digestion of walnut meal significantly diminished the mRNA expression levels of adipose synthesis-related genes.The levels of free fatty acids(FFA)decreased by 40.14%and 39.50%,respectively,while the level of glycine increased by 35.12%.Additionally,the expression levels of mRNA for genes PPAR-γand C/EBPαin adipocytes were found to be decreased by 31.42%and 55.25%,respectively,when the mass concentration of the enzymatic digest of walnut meal reached 50μg/mL.Furthermore,the enzymatic digest of walnut meal was observed to significantly reduce body mass,adipose tissue mass and food intake in mice fed a high-fat diet(HFD).The study demonstrated that the enzymatic digest of walnut meal inhibited lipid accumulation in 3T3-L1 adipocytes,and its mechanism of action was related to the inhibition of the expression of genes related to lipid synthesis.Additionally,it was found to inhibit lipid accumulation in HFD.

关 键 词：核桃粕酶解物 3T3-L1脂肪细胞 脂质积累 高脂饮食小鼠 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程]