胃癌患者EB病毒感染情况及其对癌组织p53、Bcl-2表达的影响  

EB Virus Infection in Patients with Gastric Cancer and Its Effects on the Expressions of p53 and Bcl-2 in Cancer Tissues

作　　者：刘松杰[1] 徐兵[1] 赵健 贾磊 沈裕厚[1] LIU Songjie;XU Bing;ZHAO Jian;JIA Lei;SHEN Yuhou(General Surgery Department,Xinxiang Central Hospital,Xinxiang 453000,China)

机构地区：[1]新乡市中心医院普通外科,河南新乡453000

出　　处：《河南医学研究》2024年第15期2731-2735,共5页Henan Medical Research

基　　金：河南省医学科技攻关计划联合共建项目(LHGJ20210913)。

摘　　要：目的探究胃癌患者人类疱疹(EB)病毒感染情况及其对癌组织p53、B淋巴细胞瘤-2(Bcl-2)表达的影响。方法选取新乡市中心医院2019年8月至2023年1月收治的76例胃癌患者,采用原位杂交法检测患者癌组织及癌旁组织EB病毒编码小分子RNA表达,采用逆转录聚合酶链式反应(RT-PCR)法检测患者癌组织及癌旁组织p53、Bcl-2 mRNA表达,分析EB病毒感染与胃癌临床病理特征及癌组织p53、Bcl-2表达的关系。结果76例胃癌患者癌组织标本中EB病毒阳性率为27.63%(21/76),邻近癌旁组织标本中EB病毒阳性率为4.29%(3/76),胃癌患者癌组织EB病毒阳性率高于癌旁组织(P<0.05);与EB病毒阴性的胃癌患者比,EB病毒阳性患者中病灶位于近端胃、组织浸至浆膜层、有淋巴结转移的占比较多(P<0.05);胃癌患者癌组织p53及Bcl-2 mRNA表达均高于癌旁组织(P<0.05);EB病毒感染胃癌患者癌组织p53及Bcl-2 mRNA表达均高于未感染胃癌患者(P<0.05)。结论EB病毒感染与胃癌患者近端胃病变、组织浸至浆膜层及有淋巴结转移有关,EB病毒可能通过驱动宿主p53基因甲基化来上调p53表达,与Bcl-2协同促进癌细胞生长,这些可能为临床提供胃癌诊疗评估因子及免疫治疗新靶点。Objective To explore the human Epstein-Barr(EB)virus infection in patients with gastric cancer and its effects on the expressions of p53 and B-cell lymphoma-2(Bcl-2)in cancer tissues.Methods A total of 76 patients with gastric cancer admitted to Xinxiang Central Hospital from August 2019 to January 2023 were selected.In situ hybridization was used to detect the expressions of EB virus-encoded low molecule RNA in cancer tissues and adjacent tissues,and reverse transcription polymerase chain reaction(RT-PCR)was applied to detect the mRNA expressions of p53 and Bcl-2 in cancer tissues and adjacent tissues.The relationship of EB virus infection with clinicopathological features of gastric cancer and expressions of p53 and Bcl-2 in cancer tissues was analyzed.Results The positive rate of EB virus in cancer tissue samples of 76 patients with gastric cancer was 27.63%(21/76)and was 4.29%(3/76)in adjacent tissue samples.The positive rate of EB virus in cancer tissues of patients with gastric cancer was higher than that in adjacent tissues(P<0.05).Compared with patients with EB virus-negative gastric cancer,EB virus-positive patients had high proportions of lesions in proximal stomach,tissue immersion to the serous layer and lymph node metastasis(P<0.05).The mRNA expressions of p53 and Bcl-2 in cancer tissues were higher than those in adjacent tissues(P<0.05).The mRNA expressions of p53 and Bcl-2 in cancer tissues of patients with EB virus infection were higher than those of patients without EB virus infection(P<0.05).Conclusion EB virus infection is associated with proximal gastric lesions,tissue immersion to the serous layer and lymph node metastasis in patients with gastric cancer.EB virus can up-regulate p53 expression by driving host p53 gene methylation,and promote cancer cell growth in collaboration with Bcl-2,which may provide clinical evaluation factors for gastric cancer diagnosis and treatment and new targets for immunotherapy.

关 键 词：胃癌 人类疱疹病毒 P53 B淋巴细胞瘤-2 

分 类 号：R735.2[医药卫生—肿瘤]