基于生物信息学分析纤维蛋白1在舌癌中的表达及临床意义  

Fibulin-1 expression in tongue cancer and clinical value based on bioinformatics analysis

作　　者：陈鑫如 韩瑞 徐锦程 CHEN Xinru;HAN Rui;XU Jincheng(Department of Gastrointestinal Surgery,the First Affiliated Hospital of Bengbu Medical University,Bengbu,Anhui 233004,China)

机构地区：[1]蚌埠医科大学第一附属医院口腔科,安徽蚌埠233004

出　　处：《中华全科医学》2024年第8期1317-1320,1343,共5页Chinese Journal of General Practice

基　　金：安徽省教育厅重大项目(KJ2021ZD0088);蚌埠医学院自然科学项目(2021byzd112)。

摘　　要：目的探讨舌癌组织及舌癌细胞中纤维蛋白1(FBLN1)的表达及临床意义。方法基于HPA数据库分析FBLN1在头颈部癌组织中的表达情况;GEPIA数据库和Pearson相关性分析研究FBLN1与MKI67两者在头颈部癌中表达的关联;Kaplan-Meier Plotter数据库在线分析FBLN1表达水平与头颈部癌患者生存率的关系;免疫组化和Western blotting实验验证FBLN1在舌癌组织中的表达情况;构建过表达FBLN1舌癌细胞株,通过划痕实验、Transwell实验检测FBLN1对舌癌细胞迁移、侵袭能力的影响。结果HPA数据库检索和免疫荧光显示,FBLN1在人体正常细胞和组织中高表达,在头颈部癌组织中较癌旁组织低表达(P<0.05)。GEPIA数据库分析显示,FBLN1在头颈部癌与癌旁组织中表达水平差异无统计学意义(P>0.05),而MKI67在头颈部癌中高表达(P<0.05),且Pearson相关性分析显示二者表达不相关(P=0.280)。Kaplan-Meier Plotter生存分析表明,FBLN1表达与头颈部癌总生存率无关(log-rank P=0.190),而FBLN1高表达时无病生存率更高(log-rank P=0.007)。免疫组化结果显示,FBLN1在癌旁组织中的染色强度和阳性面积均高于肿瘤组织(P<0.001)。Western blotting结果显示,在10例舌癌及配对癌旁组织中,FBLN1蛋白水平在癌旁组织中表达更高(P<0.001)。划痕实验和Transwell实验结果显示,过表达FBLN1显著抑制舌鳞癌细胞Cal-27的迁移、侵袭能力(P<0.05)。结论FBLN1基因在舌癌组织中低表达,而过表达FBLN1抑制舌鳞癌细胞Cal-27的迁移和侵袭能力,提示其可作为评价舌癌患者预后的潜在标志物。Objective To explore the expression and clinical significance of Fibulin-1(FBLN1)in tongue cancer tissue and tongue cancer cells.Methods The expression of FBLN1 in head and neck cancer tissues was analyzed based on the HPA database.The association between FBLN1 and MKI67 expression in head and neck cancer was analyzed using the GEPIA database.The relationship between FBLN1 expression levels and the survival time of head and neck cancer patients was examined using the Kaplan-Meier Plotter database.Gene enrichment pathway prediction was performed to investigate the potential biological functions of FBLN1 in head and neck cancer.Immunohistochemistry(IHC)and Western blotting were used to validate the expression of FBLN1 in tongue cancer tissues.Stable overexpression of FBLN1 in tongue cancer cells was established,scratch and Transwell chamber experiments were conducted to assess its impact on cell invasion ability.Results Analysis from the HPA database showed that FBLN1 had lower expression levels in head and neck cancer tissues compared to adjacent non-cancerous tissues.Analysis from the GEPIA database revealed no significant difference(P>0.05)in FBLN1 expression between tumor tissues and adjacent non-cancerous tissues,while MKI67 exhibited higher expression levels(P<0.05)with no correlation between their expressions(P=0.280).Survival analysis from Kaplan-Meier Plotter indicated no association between FBLN1 expression and overall survival time for tongue cancer patients(log-rank P=0.190),but higher survival rates were observed when FBLN1 was highly expressed(log-rank P=0.007).Gene enrichment pathways suggested that FBLN1 may be involved in multiple pathways related to tongue cancer development.IHC staining demonstrated that both staining intensity and positive area for Fibulin-1 were significantly higher than those observed for tumor tissue samples(P<0.00l).Western blotting showed that the level of FBLN1 protein was higher in adjacent cancer tissues compared to tongue cancer tissues in 10 cases(P<0.001).Scratch and

关 键 词：舌癌 头颈部癌 纤维蛋白1 生物信息学 细胞迁移 细胞侵袭 

分 类 号：R739.86[医药卫生—肿瘤] R730.43[医药卫生—临床医学]