嘎狗噜醇提物抗鸭乙型肝炎病毒感染的短期疗效分析  

Analysis of short term efficacy of alcohol extract of Melastoma dodecandrum Lour against duck hepatitis B virus infection

作　　者：卢良红 何沂蓉 熊国林[1] 杨路[1] 崔传宝 谢志春[1] LU Lianghong;HE Yirong;XIONG Guolin;YANG Lu;CUI Chuanbao;XIE Zhichun(School of Public Health,Guangxi Medical University,Nanning 530021,Guangxi,China)

机构地区：[1]广西医科大学公共卫生学院,广西南宁530021

出　　处：《右江医学》2024年第7期584-590,共7页Chinese Youjiang Medical Journal

基　　金：国家自然科学基金(81460512)。

摘　　要：目的探究嘎狗噜醇提物体内抗鸭乙肝病毒(DHBV)及保肝作用。方法建立鸭乙型肝炎病毒感染动物模型,随机分成6组(每组10只):嘎狗噜醇提物高剂量组、中剂量组、低剂量组,安慰剂组,拉米夫定组,非感染组,每日灌胃给药1次,持续28天;在给药前(T_(0))及给药后7 d(T_(7))、14 d(T_(14))、21 d(T_(21))、28 d(T_(28))和停药后5 d(P_(5)),取各组麻鸭静脉血,实时荧光定量PCR法检测鸭血清样本中鸭乙肝病毒(DHBV DNA)水平、酶联免疫吸附法(ELISA)测定鸭乙肝表面抗原(DHBsAg)和鸭乙肝e抗原(DHBeAg),检测ALT和AST水平,停药后6天(P_(6))处死麻鸭并解剖取肝,观察麻鸭肝脏病理切片。结果给药前(T_(0)),基线指标差异无统计学意义(P>0.05)。嘎狗噜醇提物各剂量组均未发现DHBV DNA载量下降,DHBsAg OD值也未见降低(P>0.05),对AST作用不明显;嘎狗噜醇提物各剂量组DHBeAg OD值、ALT均降低(P<0.05);肝脏病理结果显示,嘎狗噜醇提物高剂量组病变程度低于其他剂量组(P<0.05)。结论未发现嘎狗噜醇提物有抑制DHBV、DHBsAg的作用,对AST作用不明显;嘎狗噜醇提物各剂量组均能抑制DHBeAg、降低ALT水平,高剂量组能够改善肝脏病理损伤,对肝脏有一定保护作用。Objective To investigate the in vivo anti-duck hepatitis B virus(DHBV)and hepatoprotective effects of the alcohol extract of Melastoma dodecandrum Lour.Methods Animal models of DHBV infection were established.And they were randomly divided into 6 groups(with 10 ducks in each group):high,medium and low dose groups of alcohol extract of Melastoma dodecandrum Lour,placebo group,lamivudine group,and non-infected group,and intragastric administration was performed once a day for 28 days.Venous blood were collected from each group of ducks before administration(T_(0)),7 days after administration(T_(7)),14 days(T_(14)),21 days(T_(21)),28 days(T_(28))and 5 days after drug withdrawal(P 5).And then,venous blood of each group of ducks was collected to detect duck hepatitis B virus(DHBV DNA)levels in duck serum samples by real-time fluorescent quantitative PCR,and duck hepatitis B surface antigen(DHBsAg)and duck hepatitis B e antigen(DHBeAg)by enzyme-linked immunosorbent assay(ELISA).ALT and AST levels were detected,and the ducks were killed and their livers were dissected and removed 6 days after drug withdrawal(P 6),and pathological sections of their livers were observed.Results At T_(0),there was no statistically significant difference in baseline indicators(P>0.05).No decrease in DHBV DNA load or DHBsAg OD value was observed in all dose groups of alcohol extract of Melastoma dodecandrum Lour(P>0.05),and its effect on AST was not significant.The ALT and OD value of DHBeAg in each dose group of the alcohol extract of Melastoma dodecandrum Lour decreased(P<0.05).The liver pathological results showed that the degree of lesion in the high-dose group of the alcohol extract of Melastoma dodecandrum Lour was lower than that in other dose groups(P<0.05).Conclusion No inhibitory effects on DHBV and DHBsAg are found in the alcohol extract of Melastoma dodecandrum Lour,and its effect on AST is not significant.The alcohol extract of Melastoma dodecandrum Lour can inhibit DHBeAg and reduce ALT levels in all dose groups.Those in the hi

关 键 词：嘎狗噜醇提物 麻鸭 鸭乙肝病毒 拉米夫定 

分 类 号：R-332[医药卫生]