术前血清sTim-3、HMGB1水平与肌层浸润性膀胱癌根治术后预后的关系  

Relationship between preoperative serum sTim-3,HMGB1 levels and the prognosis of muscle-invasive bladder cancer after radical surgery

作　　者：薛慧英[1] 李鸿斌[1] 赵少成[1] XUE Huiying;LI Hongbin;ZHAO Shaocheng(Endoscopy Room,Datong Fifth People′s Hospital,Datong,Shanxi 037000,China)

机构地区：[1]山西省大同市第五人民医院内窥镜室,山西大同037000

出　　处：《检验医学与临床》2024年第16期2417-2421,2426,共6页Laboratory Medicine and Clinic

基　　金：山西省大同市重点研发计划项目(2019012)。

摘　　要：目的探讨术前血清可溶性T细胞免疫球蛋白黏蛋白分子-3(sTim-3)、高迁移率族蛋白B1(HMGB1)水平与肌层浸润性膀胱癌(MIBC)根治术后预后的关系。方法选取2019年6月至2020年6月该院收治的85例MIBC患者作为MIBC组,另选取同期在该院体检的85例健康者作为对照组。采用酶联免疫吸附试验(ELISA)检测所有受试者血清sTim-3、HMGB1水平;根据血清sTim-3、HMGB1水平均值将MIBC患者分为sTim-3高表达组(≥均值)和sTim-3低表达组(<均值)、HMGB1高表达组(≥均值)和HMGB1低表达组(<均值)。对比各组血清sTim-3、HMGB1水平,以及不同sTim-3、HMGB1水平与MIBC患者病理特征的关系。采用Pearson相关分析MIBC患者血清sTim-3水平与血清HMGB1水平的相关性;采用Kaplan-Meier生存曲线分析不同血清sTim-3、HMGB1水平MIBC患者的生存预后情况。结果MIBC组患者血清sTim-3、HMGB1水平高于对照组(P<0.05)。Pearson相关性分析结果显示,MIBC组患者血清sTim-3水平与血清HMGB1水平呈正相关(r=0.405,P<0.001)。不同年龄、性别、肿瘤最大径及是否发生淋巴结转移MIBC患者的血清sTim-3、HMGB1水平比较,差异均无统计学意义(P>0.05),而不同TNM分期、组织分级、淋巴结状态MIBC患者的血清sTim-3、HMGB1水平比较,差异均有统计学意义(P<0.05)。根据血清sTim-3、HMGB1水平的均值将85例MIBC患者分为sTim-3高表达组(≥3.67 ng/mL,n=44)和sTim-3低表达组(<3.67 ng/mL,n=41)、HMGB1高表达组(≥14.91 ng/mL,n=47)和HMGB1低表达组(<14.91 ng/mL,n=38)。Kaplan-Meier生存曲线结果显示,sTim-3低表达组患者的3年生存曲线高于sTim-3高表达组患者(Log-rankχ^(2)=6.175,P=0.013),HMGB1低表达组患者的3年生存曲线高于HMGB1高表达组患者(Log-rankχ^(2)=4.056,P=0.044)。sTim-3高表达组与sTim-3低表达组MIBC患者的3年生存率分别为52.27%、78.05%,HMGB1高表达组与HMGB1低表达组MIBC患者3年生存率分别为55.32%、76.31%。结论血清sTim-3、HMGB1在MIBC患�Objective To investigate the relationship between serum soluble T cell immunoglobulin domain and mucin domain-3(sTim-3)and high mobility group box 1 protein(HMGB1)levels before surgery and the prognosis of muscle-invasive bladder cancer(MIBC)after radical surgery.Methods Eighty-five MIBC patients admitted to the hospital from June 2019 to June 2020 were selected as the MIBC group,and another 85 healthy people who had a physical examination in the hospital during the same period were selected as the control group.Enzyme-linked immunosorbent assay(ELISA)was used to detect serum sTim-3 and HMGB1 levels in all subjects.MIBC patients were divided into sTim-3 high-expression group(≥mean value)and sTim-3 low-expression group(<mean value),HMGB1 high-expression group(≥mean value)and HMGB1 low-expression group(<mean value)according to the mean values of serum sTim-3 and HMGB1 levels.Serum sTim-3 and HMGB1 levels were compared between different groups,as well as the relationship between different serum sTim-3 and HMGB1 levels and clinicopathologic features of MIBC patients.Pearson correlation analysis was used to analyze the correlation between serum sTim-3 level and serum HMGB1 level in MIBC patients.Kaplan-Meier survival curves were used to analyze the survival prognosis of MIBC patients with different serum sTim-3 and HMGB1 levels.Results The serum sTim-3 and HMGB1 levels in the MIBC group were higher than those in the control group(P<0.05).The results of Pearson correlation analysis showed a positive correlation between the serum sTim-3 level and the serum HMGB1 level of patients in the MIBC group(r=0.405,P<0.001).Comparison of serum sTim-3 and HMGB1 levels in MIBC patients with different ages,genders,maximum tumor diameters and whether lymph node metastasis occurred or not showed no statistically significant differences(P>0.05),while comparison of serum sTim-3 and HMGB1 levels in MIBC patients with different TNM stages,tissue grades and lymph node status showed statistically significant differences(P<0.05).Based on

关 键 词：肌层浸润性膀胱癌 可溶性T细胞免疫球蛋白黏蛋白分子-3 高迁移率族蛋白B1 相关性 预后 

分 类 号：R737.14[医药卫生—肿瘤] R446.1[医药卫生—临床医学]