藜麦生长素结合蛋白基因ABP1的鉴定与表达分析  

Identification and characterisation of auxin-binding protein gene ABP1 in Chenopodium quinoa

作　　者：徐宏申 姜晓东[1] 王锡亮 孙慧琼 窦苗苗 王新宇 刘卓君 杨博慧 柴文婷 赵珊珊 张春来[1] XU Hongshen;JIANG Xiaodong;WANG Xiiang;SUN Huiqiong;DOU Miaomiao(WANG Xinyu,LIU Zhuojun,YANG Bohui,CHAI Wenting,ZHAO Shanshan,ZHANG Chunlai*Ministry of Education and Shanxi Province Co-Funded Collaboration and Innovation Centre for Speciality Crops,College of Agronomy,Shanxi Agricultural University,Jinzhong,Shanxi 030801,China#Co-first authors)

机构地区：[1]山西农业大学农学院,省部共建黄土高原特色作物协同创新共建中心,山西晋中030801

出　　处：《植物生理学报》2024年第7期1157-1167,共11页Plant Physiology Journal

基　　金：山西农业大学生物育种工程项目(YZGC104);黄土高原特色作物优质高效生产省部共建协同创新中心基金项目(SBGJXTZXKF-11);国家自然科学基金(31971994);山西省重点研发计划(201803D221012-2);中国科技部中巴援助项目(KY202-002002)。

摘　　要：生长素结合蛋白ABP1已被证实具有生长素受体功能。以拟南芥(Arabidopsis thaliana)的ABP1为基础,通过BLAST筛选藜麦的ABP1基因、蛋白质序列,并结合生物信息学方法对其理化性质以及亚细胞定位、二级和三级结构、蛋白序列的motif及domain、系统进化树、启动子顺式作用元件进行了分析。结果显示:藜麦基因组有2个编码ABP1基因CqABP1.7g和CqABP1.17g,位于Chr07和Chr17,等电点为6.03到6.35,蛋白质中脂肪族侧链氨基酸含量的相对值为90.88%到96.22%,且亚细胞定位在细胞质、内质网上;二级结构以延伸链、无规则卷曲和α-螺旋为主。藜麦的ABP1与甜菜的同源关系更近,在进化上出现了单双子叶的分化。CqABP1.17g在藜麦花中高水平表达,而CqABP1.7g在籽粒发育过程高水平表达;在CqABP1.17g中发现多个插入缺失(INDEL)变异,CqABP1.7g中只发现1个INDEl变异,未检测到移码突变;预测的互作蛋白有生长素外排载体、鸟嘌呤核苷酸交换因子SPIKE1、含CRIB结构域的蛋白等。该研究结果为进一步探讨CqABP1s基因功能,调控藜麦生长发育提供一定的理论基础与试验依据。The growth hormone binding protein ABP1 has been shown to function as a growth hormone re-ceptor.Based on ABP1 from Arabidopsis thaliana,we screened the ABP1 gene and protein sequences of quinoa by BLAST,and analyzed its physicochemical properties,subcellular localization,secondary and ter-tiary structure,motif and domain analysis,phylogenetic tree,and promoter cis-acting elements by bioinfor-matics methods.The results showed that:quinoa genome has two genes encoding ABP1,CqABP1.7g and CqABP1.17g,which are located in Chr07 and Chr17;the isoelectric points of CqABP1s are from 6.03 to 6.35,and the relative values of the content of aliphatic side-chain amino acids in the proteins are from 90.88%to 96.22%,and the subcellular localization is in the cytoplasm and endoplasmic reticulum.The secondary structure is dominated by extended chains,irregular curls and a-helices;the ABP1 of quinoa is more closely homologous to that of sugar beet,and evolutionarily diverged into monocotyledons.CqABP1.17g is expressed at a high level in quinoa flowers,whereas CqABP1.7g is expressed at a high level in seed development;multiple insertion deletion(INDEL)variants have been found in CqABP1.17g,only one INDEl variant was detected in CqABP1.7g,and no code-shifting mutation was detected;the predicted interacting proteins included Auxin efflux carrier,Guanine nucleotide exchange factor SPIKE 1,CRIB domain-containing protein,etc.The results of this study provide a theoretical foundation and experimental basis for further exploring the function of CqABP1s gene and regulating the growth and development of quinoa.

关 键 词：藜麦 ABP1 基因表达 系统进化 顺式作用元件 

分 类 号：S519[农业科学—作物学]