虾青素对急性大强度运动大鼠心肌损伤影响的代谢组学研究  

Metabolomic Study on the Effects of Astaxanthin on Myocardial Injury in Acute High-intensity Exercise Rats

作　　者：郭新明 王佳[1] 曹秀慧 高雅 王烨璠 吴丽君[2] GUO Xinming;WANG Jia;CAO Xiuhui;GAO Ya;WANG Yefan;WU Lijun(School of Physical Education,Taiyuan Normal University,Taiyuan 030619,China;School of Physical Education,Shanxi University,Taiyuan 030006,China)

机构地区：[1]太原师范学院体育学院,山西太原030619 [2]山西大学体育学院,山西太原030006

出　　处：《山西大学学报（自然科学版）》2024年第4期874-884,共11页Journal of Shanxi University(Natural Science Edition)

基　　金：山西省重点研发计划项目(201803D31030);教育部人文社会科学研究项目(20YJA890025)。

摘　　要：本文采用液相色谱-质谱联用技术(liquid chromatograph-mass spectrometer,LC-MS),探究补充虾青素对急性大强度运动大鼠心肌代谢的影响。选取24只7周龄健康雄性SD(Sprague Dawley)大鼠,随机分为运动组(E组)和运动给药组(EM组)。EM组灌服油溶虾青素,剂量为25 mg/(kg·d),E组以同样剂量灌胃大豆油;4周运动适应性喂养后,两组大鼠进行一次急性大强度跑台运动,然后立刻处死所有大鼠采集血液及心肌组织,血液用于检测心肌损伤标志物cTnI,心肌样品经处理后进行LC-MS检测得到对应代谢图谱,采用数据处理软件XCMS、SIMCA-P、Excel、Origin等软件进行图像和数据处理与分析,找出差异代谢物并检索Met-PA及KEGG(Kyoto Encyclopedia of Genes and Genomes)数据库,分析筛选出靶标代谢标志物及靶标代谢通路。结果显示,两组大鼠心肌差异代谢物有15种,参与了9条代谢通路,其中虾青素对急性大强度运动机体作用的潜在靶标通路有3条,分别是谷胱甘肽代谢、亚油酸代谢、半胱氨酸与蛋氨酸代谢通路。参与靶标代谢通路的标志物有6种,分别是S-腺苷蛋氨酸、3-磷酸丝氨酸、1-硬脂酰基-2-油酰基卵磷脂(1-stearoyl-2-oleoylphosphatidylcholine,SOPC)、谷胱甘肽、亚油酸、5-L-谷氨酰-L-丙氨酸,它们均与降低机体氧化应激,减少心肌损伤相关。表明虾青素主要通过调节半胱氨酸和蛋氨酸代谢、亚油酸代谢、谷胱甘肽代谢通路起到调节氧化应激,预防心肌损伤,保护心脏的作用。To investigate the effects of astaxanthin supplementation on myocardial metabolism in rats with acute high-intensity exercise by liquid chromatography-mass spectrometry(LC-MS).Twenty four seven-week-old healthy male SD(Sprague Dawley) rats were randomly divided into two exercise groups(group E) and an exercise administration group(group EM).The EM group was given oil-soluble astaxanthin(25 mg/(kg·d)) and the E group was given the same dose soybean oil.The exercise methods of both groups were:one-time acute high-intensity treadmill exercise;intervention for 4 weeks;All rats were sacrificed immediately after the last acute high-intensity exercise,and the blood and myocardial tissue was collected.The cTnI in blood,a marker of myocardial injury was detected,after sample processing,the corresponding metabolic map was detected by LC-MS technology.For example,XCMS,SIMCA-P,Excel,Origin,and other software were used for image and data processing and analysis.The differential metabolites were identified,and the target metabolic markers and metabolic pathways were screened by meT-PA and KEGG databases.The results showed that there were 15 kinds of differential metabolites in the myocardium of rats in the two groups,which were involved in 9 metabolic pathways.The glutathione metabolism,linoleic acid metabolism,cysteine,and methionine metabolism pathways were identified as three potential target pathways of astaxanthin.There were six markers involved in the target metabolic pathway:Sadenosyl methionine,serine 3-phosphate,SOPC,glutathione,and linoleic acid 5-L-glutamyl-L-alanine,they are related to the reduction of oxidative stress and myocardial injury.The results showed that astaxanthin can regulate oxidative stress,improve myocardial injury,and protect the heart by regulating the metabolism of cysteine and methionine,linoleic acid,and glutathione.

关 键 词：虾青素 运动 损伤 多组学 

分 类 号：G804.2[文化科学—运动人体科学]