线粒体核糖体蛋白L3与肺癌细胞增殖及迁移能力的关系  

Relationship between mitochondrial ribosomal protein L3 and proliferation,migration of lung cancer cells

作　　者：蒋侃[1] 黄章洲[1] 谢伟男 汪海波 周杨妹 黄韵坚[1] JIANG Kan;HUANG Zhangzhou;XIE Weinan;WANG Haibo;ZHOU Yangmei;HUANG Yunjian(Area 26,Department of Thoracic Oncology,Clinical Oncology School of Fujian Medical University/Fujian Cancer Hospital,Fuzhou 350014,Fujian,China)

机构地区：[1]福建医科大学肿瘤临床医学院/福建省肿瘤医院胸部肿瘤26区,福州3500140

出　　处：《癌症进展》2024年第13期1415-1418,1422,共5页Oncology Progress

基　　金：福建省自然科学基金(2020J011120)。

摘　　要：目的探讨线粒体核糖体蛋白L3(MRPL3)与肺癌细胞增殖及迁移能力的关系。方法从癌症基因组图谱(TCGA)数据库和在线网站UALCAN获得MRPL3在各肿瘤组织和癌旁组织中的表达水平。构建过表达MRPL3慢病毒转染H1299细胞株,构建敲低MRPL3慢病毒转染HCC827细胞株。采用定量逆转录聚合酶链反应(qRT-PCR)和蛋白质印迹法(Western blot)验证细胞株是否构建成功。采用CCK-8法检测肺癌细胞的增殖能力,Transwell细胞迁移实验检测细胞迁移能力。结果生物信息学分析显示,MRPL3在肺腺癌组织中的表达水平明显高于邻近正常组织,MRPL3在各肿瘤组织中的表达水平普遍高于癌旁组织。对体外培养肿瘤细胞进行检测,过表达MRPL3肺癌细胞的增殖及迁移能力高于对照组,敲低MRPL3肺癌细胞的增殖及迁移能力低于对照组。结论MRPL3过表达对肺癌细胞的增殖及迁移起到促进作用。Objective To investigate the relationship between mitochondrial ribosomal protein L3(MRPL3)and proliferation,migration of lung cancer cells.Method The expression levels of MRPL3 in tumor tissues and adjacent tissues were obtained from The Cancer Genome Atlas(TCGA)database and UALCAN.The overexpressing MRPL3 lentivirus transfected H1299 cell line was constructed,and the knockdown MRPL3 lentivirus transfected HCC827 cell line was constructed.Quantitative reverse transcription-polymerase chain reaction(qRT-PCR)and Western blot were used to verify the successful construction of cell lines or not.The proliferative and migration ability of lung cancer cells were detected by CCK-8 assay and Transwell cell migration assay.Result The bioinformatics analysis showed that the expression level of MRPL3 in lung adenocarcinoma tissues was higher than that in adjacent normal tissues,and the expression levels of MRPL3 in various tumor tissues were generally higher than those in adjacent tissues.The proliferation and migration ability of overexpressing MRPL3 lung cancer cells were higher than those of control group,and the proliferation and migration ability of knockdown MRPL3 lung cancer cells were lower than those of control group.Conclusion Overexpression of MRPL3 can promote the proliferation and migration of lung cancer cells.

关 键 词：线粒体核糖体蛋白L3 肺癌 增殖 迁移 

分 类 号：R734.2[医药卫生—肿瘤]