燕麦sHSP基因家族的鉴定及其响应高温及老化的表达分析  

Identification of the oat sHSP gene family and its transcript profiles in response to high temperature and aging

作　　者：马圆 刘欢[1] 赵桂琴[1] 王敬龙 张然 姚瑞瑞 MA Yuan;LIU Huan;ZHAO Gui-qin;WANG Jing-long;ZHANG Ran;YAO Rui-rui(Key Laboratory of Grassland Ecosystem,Ministry of Education,Pratacultural College,Gansu Agricultural University,Lanzhou 730070,China;Institute of Pratacultural Science,Tibet Academy of Agriculture and Animal Husbandry Sciences,Lhasa 850000,China;Institute of Ecological Protection and Restoration,Chinese Academy of Forestry,Grassland Research Center,National Forestry and Grassland Administration,Beijing 100091,China)

机构地区：[1]甘肃农业大学草业学院,草业生态系统教育部重点实验室,甘肃兰州730070 [2]西藏自治区农牧科学院草业科学研究所,西藏拉萨850000 [3]中国林业科学研究院生态保护与修复研究所,国家林业和草原局草原研究中心,北京100091

出　　处：《草业学报》2024年第8期145-158,共14页Acta Prataculturae Sinica

基　　金：草业生态系统教育部重点实验室开放课题(KLGE-2022-16);国家自然科学基金(32360344);草种创新与草地农业生态系统全国重点实验室开放基金课题(SKLHIGA202301);甘肃农业大学青年导师基金(GAU-QDFC-2023-01);西藏自治区科技计划项目(XZ202201ZY0014N);国家自然青年基金(32301491)资助。

摘　　要：小热激蛋白(sHSPs)是植物体中普遍存在的由核基因编码的一类蛋白,具有保守的ACD结构域,能够在高温、干旱以及老化等胁迫刺激中发挥重要作用。本研究利用生物信息学方法在燕麦基因组中鉴定得到24个HSP20(AsHSP20.1~AsHSP20.24)基因,并对AsHSP20家族成员的理化性质、蛋白结构、亚细胞定位、系统进化、保守基序和保守结构域、基因染色体位置以及响应高温及老化基因的表达等进行系统分析。研究发现AsHSP20基因分布在17条染色体上。编码氨基酸数目136~529 aa,分子量大小14.9~58.1 kDa,理论等电点5.30~8.79。HSP20成员多定位在核、胞质和叶绿体上,部分定位在质膜、线粒体、过氧化物酶体以及胞外。蛋白质二级结构和三级结构分析表明AsHSP20成员确定有β-折叠结构。根据保守基序组成与系统发育关系分析,AsHSP20基因家族被分为11个亚组,同一亚组成员之间具有相似或相同的保守基序,表明这些蛋白之间具有功能的相似性。进一步分析自然老化和人工老化处理下AsHSP20基因的表达情况,结果表明AsHSP20.20、AsHSP20.24基因在自然老化处理与人工老化处理下共同下调表达,推测AsHSP20.20和AsHSP20.24在两种老化方法下共同参与调控燕麦种子活力降低的过程,可作为燕麦种子寿命及抗老化种质育种研究的候选基因。研究为AsHSP20基因家族在燕麦种质老化过程中的调控机制提供了有价值的信息,也为进一步探究燕麦HSP20基因的功能及种子抗衰老分子机制提供了理论支撑。Small heat shock proteins(sHSPs)are a type of protein that is encoded by nuclear genes in plants.The sHSPs are ubiquitous in plants,contain conserved ACD domains,and play important roles in aging as well as in responses to stresses such as high temperature and drought.In this study,24 HSP20(AsHSP20.1-AsHSP20.24)genes were identified in the oat genome using bioinformatics methods.The physical and chemical properties,protein structure,subcellular localization,phylogeny,conserved motifs,and conserved domains of the putative AsHSP20 family members were determined,and the chromosomal locations of their encoding genes and gene transcription profiles in response to high temperature and aging were systematically analyzed.The AsHSP20 genes were distributed on 17 chromosomes.The genes encoded polypeptides consisting of 136-529 amino acids,with molecular weights ranging from 14.9 to 58.1 kDa,and theoretical isoelectric points ranging from 5.30 to 8.79.Most HSP20 members were predicted to localize in the nucleus,cytoplasm,or chloroplast,and a few were predicted to localize in the plasma membrane,mitochondria,peroxisome,or extracellular space.Protein secondary and tertiary structure analyses showed that AsHSP20 members haveβ-folded structures.Based on analyses of conserved motif composition and phylogenetic relationships,the AsHSP20 gene family was classified into 11 subgroups.The conserved motifs were similar or identical among the members of the same subgroup,indicative of functional similarity among proteins in the same subgroup.Further analysis of AsHSP20 transcript profiles in seeds showed that AsHSP20.20 and AsHSP20.24 were down-regulated under both natural and artificial aging conditions.It is speculated that AsHSP20.20 and AsHSP20.24 are involved in regulating the reduction in oat seed viability during aging,and are candidate genes related to oat seed longevity.These genes have potential applications in anti-aging germplasm breeding.The results of this study provide valuable information on the regulatory mechanism of

关 键 词：燕麦 种子老化 HSP20基因家族 系统进化 表达分析 

分 类 号：S544.9[农业科学—作物学]