卵巢浆液性肿瘤组织中转导蛋白β样1X相关蛋白1、睾素2表达对病人的预后价值  

Prognostic value of transducinβ-like 1X-related protein 1 and testosterone 2 expression in ovarian serous tumor tissue in patients

作　　者：李铃铃 尹翼 何爱琴[1] LI Lingling;YIN Yi;HE Aiqin(Department of Gynecology,Affiliated Cancer Hospital of Nantong University,Nantong,Jiangsu 226000,China)

机构地区：[1]南通大学附属肿瘤医院妇瘤科,江苏南通226000

出　　处：《安徽医药》2024年第9期1761-1766,I0003,共7页Anhui Medical and Pharmaceutical Journal

基　　金：江苏省南通市科技计划项目(MS22022007)。

摘　　要：目的探究卵巢浆液性肿瘤组织中转导蛋白β样1X相关蛋白1(TBL1XR1)、睾素2蛋白表达水平及其与预后的关系。方法选取2015年4月至2017年4月南通大学附属肿瘤医院102例卵巢浆液性癌病人(恶性组),50例卵巢交界性浆液性肿瘤病人(交界组),48例卵巢良性浆液性肿瘤病人(良性组)。免疫组织化学法检测组织中TBL1XR1、睾素2蛋白表达。实时荧光定量PCR检测血清TBL1XR1、睾素2 mRNA表达水平。Kaplan-Meier分析TBL1XR1、睾素2蛋白不同表达情况与生存时间关系。Cox回归模型分析恶性组病人不良预后影响因素。受试者操作特征曲线(ROC曲线)分析血清TBL1XR1、睾素2 mRNA对恶性组不良预后预测价值。结果恶性组TBL1XR1、睾素2蛋白高表达率高于交界组,交界组又高于良性组(P<0.05)。恶性组、交界组、良性组血清TBL1XR1(2.27±0.58,1.41±0.37,1.06±0.13)、睾素2 mRNA表达水平(2.66±0.67,1.58±0.40,1.09±0.15)逐次降低(P<0.05)。FIGO分期为Ⅲ~Ⅳ期、淋巴结转移及组织学分级为高级别TBL1XR1、睾素2蛋白高表达率高于Ⅰ~Ⅱ期、淋巴结无转移及低级别(P<0.05)。生存分析显示,TBL1XR1、睾素2蛋白高表达组5年累积生存率分别低于TBL1XR1、睾素2低表达组(P<0.05)。Cox多因素回归分析,FIGO分期Ⅲ~Ⅳ期、淋巴结发生转移、组织学分级为高级别、TBL1XR1高表达、睾素2高表达是卵巢浆液性癌病人不良预后危险因素(P<0.05)。恶性组死亡病人血清TBL1XR1(2.65±0.61比1.82±0.54)、睾素2 mRNA表达水平(3.10±0.76比2.15±0.57)高于生存病人(P<0.05)。ROC曲线结果显示,血清TBL1XR1、睾素2 mRNA预测恶性组病人不良预后的曲线下面积(AUC)都为0.84;二者联合预测不良预后的AUC为0.94,优于TBL1XR1、睾素2 mRNA单独预测(Z=2.24、P=0.025,Z=2.17、P=0.030)。结论卵巢浆液性癌组织及血清TBL1XR1、睾素2均为高表达,与FIGO分期、淋巴结转移、组织学分级及不良预后密切相关,血清TBL1XR1�Objective To investigate the expression levels of transducinβ-like 1X-related protein 1(TBL1XR1)and testosterone 2 in ovarian serous tumor tissues and their relationship with prognosis.Methods A total of 102 patients with ovarian serous tumor(malig-nant group),50 patients with ovarian borderline serous tumors(borderline group),and 48 patients with ovarian benign serous tumors(benign group)admitted to hospital from April 2015 to April 2017 were selected from the Affiliated Cancer Hospital of Nantong Univer-sity.Immunohistochemistry was used to detect the protein expression of TBL1XR1 and testosterone 2 in tissues.Real-time fluorescence quantitative PCR was performed to detect the serum mRNA expression levels of TBL1XR1 and testosterone 2.Kaplan-Meier analysis was performed to analyze the associations between different protein expression profiles of TBL1XR1 and testosterone 2 and the survival time.Cox regression modeling was performed to analyze the factors influencing the poor prognosis of the patients in the malignant group.Receiver operating characteristic(ROC)curves were analyzed to determine the predictive value of the serum TBL1XR1 and tes-tosterone 2 mRNA for poor prognosis in the malignant group.Results The rates of high TBL1XR1 and testosterone 2 protein expres-sion in the malignant group were greater than those in the borderline group,and the rates in the borderline group were greater than those in the benign group(P<0.05).The mRNA expression levels of serum TBL1XR1(2.27±0.58,1.41±0.37,1.06±0.13)and testoster-one 2(2.66±0.67,1.58±0.40,1.09±0.15)in the malignant group,borderline group,and benign group decreased successively(P<0.05).High-grade patients with FIGO stageⅢ-Ⅳdisease,lymph node metastasis and histological grade had higher expression levels of the TBL1XR1 and testosterone 2 proteins than patients with FIGO stageⅠ-Ⅱdisease,no lymph node metastasis and low-grade disease(P<0.05).Survival analysis revealed that the 5-year cumulative survival rate was lower in the TBL1XR1 and testosteron

关 键 词：卵巢肿瘤 转导蛋白β样1X相关蛋白1 睾素2 预后 

分 类 号：R737.31[医药卫生—肿瘤]