泛素样蛋白质2在结直肠癌中的表达及其对癌细胞增殖作用  

Expression of UBQLN2 in colorectal cancer tissues and its role in colorectal cancer cell line proliferation

作　　者：孙光源 王晓元[1] 任艳丽 尹洁[1] 胡智洁 武雪亮[1] 薛军[1] SUN Guangyuan;WANG Xiaoyuan;REN Yanli;YIN Jie;HU Zhijie;WU Xueliang;XUE Jun(Department of General Surgery,The First Affiliated Hospital of Hebei North University,Zhangjiakou,Hebei 075000,China)

机构地区：[1]河北北方学院附属第一医院普通外科,河北张家口075000

出　　处：《安徽医药》2024年第9期1810-1813,共4页Anhui Medical and Pharmaceutical Journal

基　　金：张家口市重点研发计划项目(2021073D)。

摘　　要：目的探讨泛素样蛋白质2(UBQLN2)在结直肠癌中的表达及其对结直肠癌细胞增殖的影响。方法选取2020年1月至2021年1月河北北方学院附属第一医院治疗的90例病人为研究对象,根据术中手术获取组织情况,分为正常组、癌旁组和肿瘤组。免疫组织化学法和免疫荧光评估UBQLN2在肿瘤组织、远端组织和正常组织中表达;分析UBQLN2与病人临床病理特征以及预后的相关性;蛋白质印迹法和实时荧光定量逆转录聚合酶链反应(qRT-PCR)检测UBQLN2在结直肠癌细胞系HT29、SW620、SW480、LOVO、HCT116以及人正常肠上皮细胞HIEC细胞中的表达;分别过表达和干扰UBQLN2在结肠癌细胞中的表达,随后CCK-8检测干扰前后细胞增殖能力变化。结果UBQLN2在结直肠癌癌旁组织中阳性面积(32.57±12.44)%显著高于肿瘤组织(12.68±9.79)%;UBQLN2 mRNA表达在转移阴性病人肿瘤组织中0.98±0.15显著高于转移阳性病人肿瘤组织0.33±0.08;UBQLN2的表达与结直肠癌病人预后呈正相关;UBQLN2在人正常肠上皮细胞中表达显著高于结直肠癌细胞,且在HCT116中表达最低;HCT116过表达UBQLN2培养36 h后,与pcDNA3.1-NC组细胞增殖能力0.78±0.23比较,pcDNA3.1-UBQLN-2组细胞0.52±0.18增殖能力显著降低;SW480细胞敲减UBQLN2培养36 h后,与si-NC细胞0.65±0.37增殖能力比较,si-UBQLN-2组细胞0.89±0.36增殖能力显著增加。结论UBQLN2在结直肠癌肿瘤组织和细胞中明显低表达并与转移呈负相关,UBQLN2可抑制结直肠癌增殖并与病人预后呈正相关。Objective To evaluate the expression of ubiquilin 2(UBQLN2)gene in colorectal cancer and its role in colorectal cancer cell proliferation and invasion.Methods The study included 90 patients who underwent treatment in The First Affiliated Hospital of Hebei North University from January 2020 to January 2021.Based on intraoperative tissue acquisition,the patients were assigned into three groups:normal group,para-carcinoma group,and tumor group.Immunohistochemistry and immunofluorescence techniques were used to detect the expressions of UBQLN2 in tumor tissue.distal tissue and normal tissue.The correlation between UBQLN2 expression,clinicopathological features and prognosis was analyzed.Real-time fluorescent quantitative reverse transcription polymerase chain reaction(qRT-PCR)and Western blotting were performed to measure UBQLN2 expression levels in HT29,SW620,SW480,LOVO,HCT116 and human normal intestinal epithelial cells(HIEC cells),respectively.After overexpression and interference of UBQLN2 in colon cancer cells,CCK-8 assays were used to assess cell proliferation before and after interference.Results The postive area rate of UBQLN2 in the para-carcinoma tissues of colorectal cancer was significantly higher than that in the tumor tissues[(32.57±12.44)%vs.(12.68±9.79)%].The mRNA expression level of UBQLN2 in tumor tissues from metastasis-negative patients was significantly higher than that observed in tumor tissues from metastasis-positive patients(0.98±0.15 vs.0.33±0.08).Moreover,the expression of UBQLN2 was positively correlated with patient prognosis.Analysis using qRT-PCR and Western blotting showed a higher expression of UBQLN2 in HIEC cells than in colorectal cancer cells and lowest in HCT116 cells.Thirty-six hours after UBQLN2 overexpression in HCT116 cells,the proliferation capacity of pcDNA3.1-UBQLN-2 group cells was significantly reduced compared to that of the pcDNA3.1-NC group cells(0.52±0.18 vs.0.78±0.23).Thirty-sxi hours after UBQLN2 knockdown in SW480 cells,the proliferation capacity of si-UBQLN

关 键 词：泛素类 结直肠癌 细胞增殖 生存期 预后 

分 类 号：R735.34[医药卫生—肿瘤]