UBE2C在乳腺癌MCF-7细胞增殖中的作用及机制研究  

Role and Mechanism of UBE2C in the Proliferation of MCF-7 Cells in Breast Cancer

作　　者：吴琼 贾永峰[2] WU Qiong;JIA Yongfeng(Basic Medical Col lege of Inner Mongolia Medical University,Inner Mongolia 010030,China)

机构地区：[1]内蒙古医科大学基础医学院,呼和浩特010030 [2]内蒙古医科大学病理学教研室,呼和浩特010030

出　　处：《医学研究杂志》2024年第7期73-78,共6页Journal of Medical Research

基　　金：内蒙古自治区科技计划项目(2019GG083)。

摘　　要：目的研究沉默泛素结合酶E2C(ubiquitin-conjugating enzyme2C,UBE2C)对人乳腺癌MCF-7细胞增殖能力的影响,并初步研究其作用机制。方法利用小干扰RNA(si-RNA)沉默MCF-7细胞中UBE2C基因。利用CCK-8实验及克隆形成实验检测细胞增殖能力。实时荧光定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-qPCR)、Western blot法分别在mRNA、蛋白水平检测细胞周期相关蛋白激酶抑制蛋白p21以及细胞周期促进蛋白细胞周期蛋白D1(cellcycle protein D1,CyclinD1)、细胞周期蛋白依赖性激酶4(cyclin-dependent kinase4,CDK4)、细胞周期蛋白依赖性激酶6(cyclin-depend-ent kinase 6,CDK6)的表达。结果与空白对照组及阴性对照(si-NC)组细胞比较,si-UBE2C组细胞UBE2C表达下调。CCK-8实验以及克隆形成实验结果显示,si-UBE2C组细胞增殖能力明显降低;与空白对照组及si-NC组细胞比较,si-UBE2C组细胞周期相关蛋白激酶抑制蛋白p21在mRNA及蛋白水平的表达均上调,而细胞周期促进蛋白CyclinD1、CDK4、CDK6在mRNA及蛋白水平的表达均下调。结论UBE2C可以通过介导CyclinD1/CDK4/CDK6复合物及p21的表达来调控MCF-7细胞的增殖能力,促进乳腺癌的进展。Objective To study the effect of silencing ubiquitin-conjugating enzyme 2C(UBE2C)on the proliferative capacity of human breast cancer cells MCF-7 and to preliminarily investigate its mechanism of action.Methods Silencing of UBE2C gene in MCF-7 cells using small interfering RNA(si-RNA).CCK-8 assay and clone formation assay were used to detect cell proliferation a-bility.The expression of cell cycle-associated protein kinase inhibitor protein p21 as well as cell cycle-promoting proteins CyclinD1,cyclin-dependent kinase 4(CDK4),and cyclin-dependent kinase 6(CDK6)were detected by real-time quantitative polymerase chain reaction(RT-qPCR)and Western blot at the mRNA and protein levels,respectively.Results Compared with the cells in blank control group and negative control(si-NC)group,the expression of UBE2C was down-regulated in cells of si-UBE2C group.The re-sults of CCK-8 assay and clone formation assay showed that the proliferative ability of cells in si-UBE2C group was significantly de-creased.Compared with the cells in blank control group and si-NC group,the expression of cell cycle-related protein kinase inhibitor protein p21 in the cells of si-UBE2C group was up-regulated at both the mRNA and protein levels,while the expression of cell cycle-promoting proteins CyclinDI,CDK4 and CDK6 was down-regulated at both the mRNA and protein levels.Conclusion UBE2C can reg-ulate the proliferative capacity of MCF-7 cells and promote breast cancer progression by mediating the expression of CyclinD1/CDK4/CDK6 complex and p21.

关 键 词：泛素结合酶E2C 乳腺癌 CYCLIND1 增殖 

分 类 号：R737.9[医药卫生—肿瘤]