柴术理胃饮对功能性消化不良大鼠脑-肠轴功能及胃肠动力的影响  

Effects of Chaizhu Liwei Drinking on brain gut axis function and gastrointestinal motility in functional dyspepsia rats

作　　者：范明明 常雨 张艮霜 林伟 韩海瑞 齐晶 王顺 FAN Mingming;CHANG Yu;ZAHNG Genshuang;LIN Wei;HAN Hairui;QI Jing;WANG Shun(Department of Geriatrics,Heilongjiang Academy of Traditional Chinese Medicine,Harbin,Heilongjiang 150000;Department of Acupuncture and Moxibustion,Heilongjiang Academy of Traditional Chinese Medicine,Harbin,Heilongjiang 150000)

机构地区：[1]黑龙江省中医药科学院老年病科,黑龙江哈尔滨150000 [2]黑龙江省中医药科学院针灸科,黑龙江哈尔滨150000

出　　处：《河北中医》2024年第8期1305-1310,共6页Hebei Journal of Traditional Chinese Medicine

基　　金：黑龙江省自然科学基金项目(编号:LH2023H079)。

摘　　要：目的观察柴术理胃饮对功能性消化不良(FD)大鼠脑-肠轴功能及胃肠动力的影响。方法通过矿场实验选取SPF级雄性Sprague-Dawley大鼠60只,其中50只采用郭氏夹尾刺激法+慢性疲劳+饮食失节的多重因素造模方法制备FD肝郁脾虚模型。造模成功后随机分为模型组、柴术理胃饮低、中、高剂量组和多潘立酮组,每组10只,并给予不同治疗方案,持续21 d。实验结束后观察并计算FD大鼠进食量、糖水消耗量、体质量变化,胃排空率、小肠推进率的变化,并检测大鼠血清5-羟色胺(5-HT)、一氧化氮(NO)及一氧化氮合酶(NOS)水平。最后取胃窦组织,经苏木素-伊红(HE)染色法观察各组大鼠胃窦组织病理学改变。结果造模之初大鼠均未见异常,随着造模程序的推进,除正常组大鼠外,其余各组大鼠情绪由稳定变为烦躁、易怒、易惊,最后转化为情绪低落,并逐渐在夹尾时叫声微弱;大鼠毛发由润泽变为枯黄毛躁并散乱易掉落;大鼠饮水量、进食量、糖水消耗均减少,体质量逐渐减轻;活动量减少,嗜睡少动,扎堆,不活跃,弓背蜷缩,对外界声音不敏感;大便稀溏不成形。在3周的治疗后,各治疗组一般情况逐渐恢复造模前的状态。造模后,与正常组比较,模型组大鼠进食量、体质量及糖水消耗量均降低(P<0.05)。治疗后,与模型组比较,多潘立酮组和柴术理胃饮低、中、高剂量组大鼠体质量、进食量、糖水消耗量均升高(P<0.05);柴术理胃饮高剂量组大鼠体质量、进食量、糖水消耗量均高于柴术理胃饮低剂量组(P<0.05)。与正常组比较,模型组大鼠胃排空率及小肠推进率均降低(P<0.05);与模型组比较,多潘立酮组和柴术理胃饮低、中、高剂量组大鼠胃排空率及小肠推进率均有不同程度提高(P<0.05);柴术理胃饮高剂量组胃排空率及小肠推进率均高于柴术理胃饮低剂量组(P<0.05)。与正常组比较,模型组大鼠血清NO、NOS含�Objective The purpose of this experiment is to observe the effects of Chaizhu Liwei Drinking on brain gut axis function and gastrointestinal motility in rats with functional dyspepsia(FD).Methods Sixty male specific-pathogen free(SPF)-grade Sprague-Dawley(SD)rats were selected through the field experiment.The FD model of liver depression and spleen deficiency was established by Guo's tail clipping stimulation+chronic fatigue+eating disorder.After successful modeling,the rats were divided into model group,Chaizhu Liwei Drinking low,medium and high dose group and Domperidone group,with 10 rats in each group.Different treatment schemes were given for 21 days.After the experiment,the changes in food intake,sugar-water consumption,body mass(BM),gastric emptying rate and intestinal propulsion rate of FD rats were observed and calculated,and the levels of 5-hydroxytryptamine(5-HT),nitric oxide(NO)and nitric oxide synthase(NOS)in serum were measured.Finally,the histopathological changes in gastric antrum were observed by hematoxylin eosin staining(HE staining)between groups.Results Compared with those of the normal group,the food intake,BM and sugar-water consumption of rats in the model group before modeling were significantly lower(P<0.05).After treatment,compared with the model group,the BM,food intake,and sugar-water consumption of rats in Domperidone group and Chaizhu Liwei Drinking dose groups were significantly increased(P<0.05),which in Chaizhu Liwei Drinking high dose group were significantly higher than those in Chaizhu Liwei Drinking low dose group(P<0.05).Compared with the normal group,the gastric emptying rate and intestinal propulsion rate in the model group were significantly decreased(P<0.05).Compared with the model group,which in Domperidone group and Chaizhu Liwei Drinking dose groups were significantly increased(P<0.05),which in Chaizhu Liwei Drinking high-dose group were significantly higher than those of Chaizhu Liwei Drinking low-dose group(P<0.05).Compared with the normal group,NO and NOS in the mo

关 键 词：功能性消化不良 大鼠 Sprague-Dawley 动物实验 中药疗法 5-羟色胺 一氧化氮 一氧化氮合酶 

分 类 号：R57[医药卫生—消化系统] R285.5[医药卫生—内科学]