酶促中药-壳聚糖复合水果保鲜膜研制  

Study on Preparation of Enzymatic Chinese Traditional Medicine Chitosan Composite Fruit Preservation Film

作　　者：陈宛琦 陆淼婷 黄婷婷 薛蓉 王今朝 柯德森[1] CHEN Wan-qi;LU Miao-ting;HUANG Ting-ting;XUE Rong;WANG Jin-zhao;KE De-sen(School of Life Sciences,Guangzhou University,Guangdong Guangzhou 510006;South China Botanical Garden,Guangdong Guangzhou 510650,China)

机构地区：[1]广州大学生命科学学院,广东广州510006 [2]中国科学院华南植物园,广东广州510650

出　　处：《广州化工》2024年第13期30-35,共6页GuangZhou Chemical Industry

基　　金：2022年度广州大学大学生创新训练项目(No:202211078118);广州大学生命科学学院第三届“科创杯”项目。

摘　　要：辣根过氧化物酶(Horseradish Peroxidase, HRP)有催化聚合活性强、效率高等优良特性。HRP催化的中药-壳聚糖复配膜应用于水果保鲜可减弱呼吸作用,有低失重率、高VC和可滴定酸保持率,且HRP浓度在750 U/mL时对沙糖桔保鲜效果最佳,该复配膜的施用还能影响沙糖桔可溶性蛋白和可溶性糖的含量、抗氧化性以及糖代谢不同途径。电镜表征结果提示HRP酶促聚合增强中药-壳聚糖复配物的均一性、减少了杂质。这种酶促中药-壳聚糖复配物对比普通保鲜材料具有更佳保鲜效果。Horseradish Peroxidase(HRP)has excellent properties of catalytic synthesis of polymer with strong catalytic activity and high efficiency.It was found that HRP catalyzed TCM-chitosan(Traditional Chinese Medicinechitosan)composite films can weaken the respiratory intensity,have low weight loss rate,high VC and titrable acid retention rate,and the preservation effect of Citrus tachibana is the best when the HRP concentration is 750 U/mL.The contents of soluble protein and soluble sugar,antioxidant properties and different ways of carbohydrate metabolism of Citrus tachibana were affected by the application of HRP catalyzed TCM-chitosan composite films.The results of scanning electron microscope indicated that HRP enzymatic polymerization enhanced the uniformity of the compound and reduced the impurity.The TCM-chitosan compound catalyzed by HRP had better preservation effect than ordinary preservation materials.

关 键 词：辣根过氧化物酶 中药 水果 保鲜 壳聚糖 

分 类 号：Q814.9[生物学—生物工程]