Müller cells are activated in response to retinal outer nuclear layer degeneration in rats subjected to simulated weightlessness conditions  

作　　者：Yuxue Mu Ning Zhang Dongyu Wei Guoqing Yang Lilingxuan Yao Xinyue Xu Yang Li Junhui Xue Zuoming Zhang Tao Chen 

机构地区：[1]Aerospace Clinical Medical Center,School of Aerospace Medicine,Air Force Medical University,Xi’an,Shaanxi Province,China [2]Department of Aviation Medicine,Xijing Hospital,Air Force Medical University,Xi’an,Shaanxi Province,China [3]Department of Emergency Medicine,Wuhan No.1 Hospital,Wuhan,Hubei Province,China [4]Third Regiment,School of Basic Medicine,Air Force Medical University,Xi’an,Shaanxi Province,China [5]Fourth Regiment,School of Basic Medicine,Air Force Medical University,Xi’an,Shaanxi Province,China

出　　处：《Neural Regeneration Research》2025年第7期2116-2128,共13页中国神经再生研究（英文版）

基　　金：supported by the Army Laboratory Animal Foundation of China,No.SYDW[2020]22(to TC);the Shaanxi Provincial Key R&D Plan General Project of China,No.2022SF-236(to YM);the National Natural Science Foundation of China,No.82202070(to TC)。

摘　　要：A microgravity environment has been shown to cause ocular damage and affect visual acuity,but the underlying mechanisms remain unclear.Therefore,we established an animal model of weightlessness via tail suspension to examine the pathological changes and molecular mechanisms of retinal damage under microgravity.After 4 weeks of tail suspension,there were no notable alterations in retinal function and morphology,while after 8 weeks of tail suspension,significant reductions in retinal function were observed,and the outer nuclear layer was thinner,with abundant apoptotic cells.To investigate the mechanism underlying the degenerative changes that occurred in the outer nuclear layer of the retina,proteomics was used to analyze differentially expressed proteins in rat retinas after 8 weeks of tail suspension.The results showed that the expression levels of fibroblast growth factor 2(also known as basic fibroblast growth factor)and glial fibrillary acidic protein,which are closely related to Müller cell activation,were significantly upregulated.In addition,Müller cell regeneration and Müller cell gliosis were observed after 4 and 8 weeks,respectively,of simulated weightlessness.These findings indicate that Müller cells play an important regulatory role in retinal outer nuclear layer degeneration during weightlessness.

关 键 词：glial fibrous acidic protein GLIOSIS Müller cells nerve growth factor neural differentiation neurodegeneration proteomic retinal degeneration retinal outer nuclear layer simulated weightlessness 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]