卵清蛋白-羧甲基魔芋葡甘聚糖复合物稳定的姜黄素Pickering乳液及其肠道递送性能  

Curcumin-Loaded Pickering Emulsion Stabilized by Ovalbumin-Carboxymethyl Konjac Glucomannan Complex and Its Intestinal Delivery Performance

作　　者：张译文 顾春晔 刘雪玲 曹亚倩 肖军霞[1,2] ZHANG Yiwen;GU Chunye;LIU Xueling;CAO Yaqian;XIAO Junxia(College of Food Science and Engineering,Qingdao Agricultural University,Qingdao 266109,Shandong,China;Qingdao Special Food Research Institute,Qingdao Agricultural University,Qingdao 266109,Shandong,China)

机构地区：[1]青岛农业大学食品科学与工程学院,山东青岛266109 [2]青岛农业大学特种食品研究院,山东青岛266109

出　　处：《食品研究与开发》2024年第16期41-48,共8页Food Research and Development

基　　金：国家级、省级大学生创新创业训练计划项目(202210435042)。

摘　　要：为探究羧甲基魔芋葡甘聚糖(carboxymethyl konjac glucomannan,CMKGM)在姜黄素结肠靶向递送体系构建中的应用潜力,以CMKGM与卵清蛋白(ovalbumin,OVA)形成的聚电解质复合物为稳定剂制备姜黄素Pickering乳液,研究两者质量比和总聚合物浓度对所得Pickering乳液稳定性、外观结构、姜黄素包封率和载药量的影响,并对最佳乳液在模拟胃肠液消化过程中姜黄素释放率、乳滴粒径和形态的变化规律进行跟踪。结果表明,当OVA与CMKGM质量比为1∶1、总聚合物浓度为0.4%时所得Pickering乳液的稳定性最好且姜黄素的包封率和载药量最高,分别达到了91.79%和1.05 mg/g。体外模拟消化研究表明,与OVA稳定的姜黄素乳液相比,该Pickering乳液在模拟胃液中具有更好的稳定性,且在模拟结肠液中的姜黄素释放率高达22.78%,远高于OVA稳定乳液的5.35%,表现出了良好的结肠靶向递送性能。To explore the potential of carboxymethyl konjac glucomannan(CMKGM)in the construction of colon-targeted delivery systems for curcumin,the ovalbumin(OVA)-CMKGM polyelectrolyte complex was used as a stabilizer to fabricate curcumin-loaded Pickering emulsion.The effects of the mass ratio of OVA and CMKGM as well as total biopolymer concentration on the stability,appearance,curcumin encapsulation efficiency,and drug loading were investigated.The curcumin release,particle size variation,and morphology variation patterns of the optimum emulsion in the simulated gastrointestinal fluids were tracked.The results indicated that the OVA to CMKGM mass ratio of 1∶1 and total biopolymer concentration of 0.4%conferred the highest curcumin encapsulation efficiency and drug loading of 91.79%and 1.05 mg/g respectively.In vitro simulated digestion tests revealed that compared with the curcumin emulsion stabilized by OVA,the Pickering emulsion exhibited better stability in the simulated gastric fluid.Its curcumin release rate was up to 22.78%in the simulated colonic fluid,much higher than that of the OVA-stabilized emulsion(5.35%),validating its excellent colon-targeted delivery performance.

关 键 词：姜黄素 羧甲基魔芋葡甘聚糖 卵清蛋白 聚电解质复合物 结肠递送体系 Pickering乳液 

分 类 号：TS201.2[轻工技术与工程—食品科学]