lncRNA AL365181.2在胆管癌中的表达及生物学特性  

Expression and biological characteristics of long non-coding RNA AL365181.2 in cholangiocarcinoma

作　　者：王钦磊 孙兆伟 郭敬允 李浩然 冯玉杰[1] 张炳远[1] WANG Qinlei;SUN Zhaowei;GUO Jingyun;LI Haoran;FENG Yujie;ZHANG Bingyuan(Department of Hepatobiliary and Pancreatic Surgery,The Affiliated Hospital of Qingdao University,Qingdao 266003,China)

机构地区：[1]青岛大学附属医院肝胆外科,山东青岛266003

出　　处：《精准医学杂志》2024年第4期314-319,共6页Journal of Precision Medicine

基　　金：国家自然科学基金项目(81272362)。

摘　　要：目的探究长链非编码RNA(lncRNA)AL365181.2在胆管癌(cholangiocarcinoma,CHOL)中的表达水平及其对CHOL细胞功能学的影响。方法对肿瘤基因组图谱(TCGA)数据库中有关CHOL患者癌组织和癌旁组织的lncRNA AL365181.2表达情况的资料进行比较,对lncRNA AL365181.2表达水平与CHOL组织免疫细胞浸润情况的关系进行分析,并对lncRNA AL365181.2进行GSEA和KEGG富集分析。通过EdU实验、划痕实验、Transwell实验和裸鼠成瘤实验分别检测lncRNA AL365181.2对CHOL细胞增殖、迁移、侵袭和成瘤能力的影响。结果对TCGA数据库所涉资料的分析结果显示,与癌旁组织相比,CHOL组织中lncRNA AL365181.2显著高表达(Z=4.00,P<0.05),且lncRNA AL365181.2与CHOL组织的记忆T细胞及树突状细胞浸润水平显著相关(r=0.28、-0.34,P<0.05);GSEA分析结果显示lncRNA AL365181.2在CHOL细胞免疫调节相关通路中显著富集,KEGG富集分析结果显示lncRNA AL365181.2在细胞因子受体互作通路和血管壁细胞表面互作通路中显著富集。体内外实验结果显示,敲低lncRNA AL365181.2后,CHOL细胞的增殖、迁移、侵袭及体内成瘤能力均显著降低(t=4.23~173.34,P<0.05)。结论lncRNA AL365181.2在CHOL组织中较癌旁组织中高表达,并且表达水平与免疫细胞浸润程度密切相关;lncRNA AL365181.2的表达水平与CHOL细胞的增殖能力、迁移能力和侵袭能力密切相关。Objective To investigate the expression level of long non-coding RNA(lncRNA)AL365181.2 in cholangiocarcinoma(CHOL)and its effect on the functional characteristics of CHOL cells.Methods The Cancer Genome Atlas(TCGA)database was used to compare the expression level of lncRNA AL365181.2 between cancerous tissue and paracancerous tissue in CHOL patients,and the association between the expression level of lncRNA AL365181.2 and immune cell infiltration in CHOL tissue was analyzed.The Gene Set Enrichment Analysis(GSEA)and the Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis were performed for lncRNA AL365181.2.EdU assay,wound healing assay,Transwell assay,and xenograft tumor formation experiment were used to investigate the impact of lncRNA AL365181.2 on the proliferation,migration,invasion,and tumorigenic abilities of CHOL cells.Results The analysis of TCGA data showed that the expression level of lncRNA AL365181.2 in CHOL tissue was significantly higher than that in paracancerous tissue(Z=4.00,P<0.05),and lncRNA AL365181.2 was significantly associated with the infiltration of memory T cells and dendritic cells in CHOL tissue(r=0.28,-0.34,P<0.05).The GSEA analysis showed that lncRNA AL365181.2 was significantly enriched in immune regulation pathways in CHOL cells,while the KEGG enrichment analysis showed significant enrichment of lncRNA AL365181.2 in cytokine receptor interaction pathways and cell surface interactions at the vascular wall.In vitro and in vivo experiments showed that after the knockdown of lncRNA AL365181.2,there were significant reductions in the proliferation,migration,invasion,and tumorigenic abilities of CHOL cells(t=4.23-173.34,P<0.05).Conclusion lncRNA AL365181.2 is highly expressed in CHOL tissue compared with paracance-rous tissue,and its expression level is closely associated with the degree of immune cell infiltration.In addition,the expression level of lncRNA AL365181.2 is closely associated with the proliferation,migration,and invasion abilities of CHOL cells.

关 键 词：胆管肿瘤 RNA 长链非编码 基因表达 细胞增殖 细胞运动 肿瘤浸润 致癌作用 

分 类 号：R735.8[医药卫生—肿瘤]