水苏糖及其代谢产物的高效液相色谱-蒸发光散射法检测方法  

Determination of stachyose and its metabolites by high-performance liquidchromatography-evaporative light-scattering detector

作　　者：金钰莹 钟先锋 徐家豪 郑剑玮 王阿利 陈梓琦 任红 陈忻 卢怀裕 黄桂东 JIN Yuying;ZHONG Xianfeng;XU Jiahao;ZHENG Jianwei;WANG Ali;CHEN Ziqi;REN Hong;CHEN Xin;LU Huaiyu;HUANG Guidong(Department of Food Science and Engineering,Foshan University,Foshan 528000,China;Guangdong Traditional Fermented Food Engineering Technology Research Center,Foshan 528000,China;Guangdong Food Circulation Safety Control Engineering Technology Research Center,Foshan 528000,China;Foshan Brewing Technology Engineering Research Center,Foshan 528000,China;Foshan Agricultural Biomanufacturing Engineering Technology Research Center,Foshan 528000,China;School of Environmental and Chemical Engineering,Foshan University,Foshan 528231,China;Foshan Nanhai Shimen Experimental School,Foshan 528225,China)

机构地区：[1]佛山科学技术学院食品科学与工程学院,广东佛山528000 [2]广东省传统发酵食品工程技术研究中心,广东佛山528000 [3]广东省食品流通安全控制工程技术研究中心,广东佛山528000 [4]佛山酿造技术工程技术研究中心,广东佛山528000 [5]佛山农业生物制造工程技术研究中心,广东佛山528000 [6]佛山科学技术学院环境与化学工程学院,广东佛山528231 [7]佛山市南海石门实验学校,广东佛山528225

出　　处：《食品与发酵工业》2024年第16期293-298,共6页Food and Fermentation Industries

基　　金：国家自然科学基金项目(32072198,32372284,42006125,32101926,32302023);广东省自然科学基金项目(2021B1515120042,2023A1515012536,2022A1515140021,2022A1515140021);广东省教育厅项目(2020ZDZX1046,2020ZDZX1029,2021KCXID034,2018GXJK198,2023KTSCX131)。

摘　　要：水苏糖作为一种天然益生元,可促进乳酸菌增殖,但水苏糖在乳酸菌中的代谢途径仍未被完全解析。该研究尝试建立一种检测水苏糖及其代谢产物的高效液相色谱-蒸发光散射(high performance liquid chromatography-evaporative light-scattering detector, HPLC-ELSD)方法,通过水苏糖及其代谢产物含量的动态变化为阐释水苏糖在乳酸菌中的代谢途径提供技术支撑。结果表明,使用Waters BEH Amide色谱柱结合ELSD检测器,洗脱条件为V(乙腈)∶V(水)=80∶20、流动相流速为1.0 mL/min, ELSD检测器条件为温度60℃,载气流速1.0 mL/min时,水苏糖及其代谢产物能实现较好分离,且检测重现性好,稳定性高,说明该方法适用于水苏糖及其代谢产物含量的检测。Stachyose,as a natural prebiotic,can promote the proliferation of lactic acid bacteria,but the metabolic pathway of stachyose in lactic acid bacteria is still completely resolved.This study attempts to establish a high-performance liquid chromatography-evaporative light-scattering detector(HPLC-ELSD)method for the detection of stachyose and its metabolites.The dynamic changes of stachyose and its metabolites were intended to provide technical support for the interpretation of the metabolic pathway of stachyose in lactic acid bacteria.Results showed that using Waters BEH Amide chromatographic column combined with ELSD detector,the elution conditions were acetonitrile(80%)∶water(20%),the flow rate of mobile phase was 1.0 mL/min,the temperature of ELSD detector was 60℃,and the flow rate of carrier gas was 1.0 mL/min.Stachyose and its metabolites can be well separated,and the detection reproducibility is good and the stability is high,indicating that this method can be used for the detection of stachyose and its metabolites.

关 键 词：水苏糖 代谢产物 高效液相色谱 蒸发光散射 

分 类 号：TS201.3[轻工技术与工程—食品科学] O657.72[轻工技术与工程—食品科学与工程]