牙釉基质衍生物促进人工根面牙本质龋的再矿化  

Enamel matrix derivative improves remineralization of artificial root dentin caries in vitro

作　　者：陈进 梁静鸥 张凌琳[4] CHEN Jin;LIANG Jing’ou;ZHANG Linglin(Outpatient Department of Third Affiliated Hospital,College of Stomatology,Chongqing Medical University,Chongqing,401120;Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences,Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education,Chongqing,401147;Department of Pediatric Dentistry,West China Hospital of Stomatology,Sichuan University,Chengdu,Sichuan Province,610041,China;Department of Dentistry and Endodontics,West China Hospital of Stomatology,Sichuan University,Chengdu,Sichuan Province,610041,China;State Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases,West China Hospital of Stomatology,Sichuan University,Chengdu,Sichuan Province,610041,China)

机构地区：[1]重庆医科大学附属口腔医院附三院门诊部,重庆5401120 [2]重庆市口腔疾病与生物医学重点实验室,重庆市高校市级口腔生物医学工程重点实验室,重庆401147 [3]四川大学华西口腔医院儿童口腔科,成都610041 [4]四川大学华西口腔医院牙体牙髓科,成都610041 [5]四川大学华西口腔医院口腔疾病防治全国重点实验室,国家口腔医学中心,国家口腔疾病临床医学研究中心,成都610041

出　　处：《陆军军医大学学报》2024年第16期1890-1896,共7页Journal of Army Medical University

基　　金：国家自然科学基金面上项目(81271128)。

摘　　要：目的探讨牙釉基质衍生物(enamel matrix derivative,EMD)对人工根面牙本质龋再矿化作用的影响。方法将牛牙根面牙本质样本放置在脱矿溶液中,形成人工龋损样本,采用简单随机抽样法分为EMD组(30 g/L EMD,实验组)、氟化钠(sodium fluoride,NaF)组(1 g/L NaF,阳性对照组)和去离子水(distilled and deionised water,DDW)组(阴性对照组),进行pH循环处理8 d,每天6次。使用扫描电子显微镜(scanning electron microscope,SEM)及偏振光显微镜(polarized light microscopy,PLM)观察各组处理前后龋损形态学的变化情况,并使用横断显微照相技术(transverse microradiography,TMR)检测处理前后矿物质丢失量、龋损深度和矿物含量的变化情况。结果pH循环处理后,SEM的表面形态学图像和PLM的垂直形态学图像均显示,EMD组和NaF组人工根面牙本质龋的矿物质沉积增加,而DDW组无矿物质沉积。同时,TMR结果显示,与DDW组相比,EMD组矿物丢失较低,龋损深度较浅,矿物含量较高(P<0.05)。结论釉基质衍生物能促进根面牙本质龋的再矿化,具有成为一种有效的根面无创防龋制剂的潜力。Objective To determine the effect of enamel matrix derivative(EMD)on remineralization of artificial root dentin caries.Methods Bovine root dentin blocks were placed in a demineralization solution to create artificial root dentin carious lesions.The lesions were then randomly divided into EMD group(30 g/L EMD,the experimental group),sodium fluoride(NaF)aqueous solution group(1 g/L NaF,the positive control group)and distilled and deionised water(DDW)group(the negative control group),and all the lesions were pH-cycled at 6 cycles per day for 8 d.Morphology of the lesions were observed by scanning electron microscopy(SEM)and polarized light microscopy(PLM).Transverse microradiography(TMR)was used to examine mineral loss,lesion depth and mineral content.Results After pH-cycling,the surface morphology by SEM and the vertical morphology by PLM suggested that EMD and NaF treatment,but not DDW,caused mineral accumulation of artificial root dentin caries.Meanwhile,TMR revealed significantly lower mineral loss,shallower lesion depth and more mineral content in the EMD group than the DDW group(P<0.05)after pH-cycling.Conclusion EMD can significantly promote remineralization of root dentin caries in vitro,and is a promising agent for non-invasive root caries therapy.

关 键 词：牙釉基质衍生物 根面龋 根面牙本质 再矿化 

分 类 号：R780.2[医药卫生—口腔医学] R781.1[医药卫生—临床医学] R977.6