肾癌患者中外周血淋巴细胞亚群、T-reg、MDSC的表达及临床意义  

Expression levels of lymphocyte subsets,Tregs and MDSCs in peripheral blood of patients with renal carcinoma and their clinical significances

作　　者：李涛[1] 倪晓辰[1] 张爱莉[1] LI Tao;NI Xiaochen;ZHANG Aili(The Fourth Hospital of Hebei Medical University,Hebei,Shijiazhuang 050000,China)

机构地区：[1]河北医科大学第四医院,石家庄市050000

出　　处：《河北医药》2024年第16期2417-2422,共6页Hebei Medical Journal

摘　　要：目的 采用流式细胞术分析肾癌患者MDSC、T-reg、CD_(3)^(+)CD4^(+)T细胞、CD_(3)^(+)CD_(8)^(+)T细胞和CD3-CD_(1)^(+)6CD_(5)^(+)6细胞的表达情况,以及各项指标与肾癌进展的相关性,探讨各项指标在肾癌治疗预后中的相关价值。方法 选取2021年9月至2022年11月住院的肾癌患者44例为肾癌组,选取29例健康人作为对照组。清晨,空腹采集3 mL静脉血。所有肾癌患者均已通过组织病理学诊断。采用全血9色荧光11参数流式细胞术检测肾癌患者和健康体检者MDSC、Treg、CD_(3)^(+)CD_(4)^(+)T细胞、CD_(3)^(+)CD_(8)^(+)T细胞、CD_(3)^(+)CD_(4)^(+)T细胞/CD_(3)^(+)CD_(8)^(+)T细胞比值和CD3-CD_(1)^(+)6CD_(5)^(+)6细胞表达水平。收集患者的临床数据:包括年龄、性别、BMI、临床分期、肿瘤大小、病理类型。结果 肾癌组与对照组之间性别、年龄、BMI差异均无统计学意义(P>0.05)。肾癌组与对照组之间外周血CD_(3)^(+)CD_(4)^(+)、CD_(3)^(+)CD_(8)^(+)、CD_(3)^(+)CD_(4)^(+)/CD_(3)^(+)CD_(8)^(+)、NK、T-reg、PMN-MDSC、M-MDSC水平差异有统计学意义(P<0.05)。肾癌患者CD_(3)^(+)CD_(4)^(+)、CD_(3)^(+)CD_(8)^(+)的表达水平低于对照组(P<0.05),T-reg、PMN-MDSC、M-MDSC的表达水平高于对照组(P<0.05)。肾癌周血中患者外的CD_(3)^(+)CD_(4)^(+)、CD_(3)^(+)CD_(8)^(+)、CD_(3)^(+)CD_(4)^(+)/CD_(3)^(+)CD_(8)^(+)、NK、T-reg、PMN-MDSC、M-MDSC的表达水平与性别、年龄、BMI、病理学类型水平无显著相关(P>0.05)。CD_(3)^(+)CD_(8)^(+)T、CD_(3)^(+)CD_(4)^(+)/CD_(3)^(+)CD_(8)^(+)、NK与肿瘤大小、临床分期之间无明显相关性(P>0.05),CD_(3)^(+)CD_(4)^(+)、M-MDSC、PMN-MDSC、Treg与肿瘤大小、临床分期有相关性(P<0.05)。经Logistic回归分析结果显示:外周血CD_(3)^(+)CD_(4)^(+)淋巴细胞低表达,T-reg、PMN-MDSC、M-MDSC的高表达可能与肾癌患者分期相关(P<0.05)。通过构建ROC曲线结果显示:按照临床分期进行分组,“0”为Ⅰ期、Ⅱ期组,“1”为,Ⅲ期、�Objective To measure the expression levels of myeloid-derived suppressor cells(MDSCs),regulatory T-cells(Tregs),CD_(3)^(+)CD_(4)^(+)T cells,CD_(3)^(+)CD_(8)^(+)T cells and CD_(3)^(+)CD16^(+)CD56^(+)T cells in renal carcinoma patients by flow cytometry,and to identify their correlation with the progression of renal carcinoma and their clinical significance.Methods From September 2021 to November 2022,44 renal carcinoma patients hospitalized in our hospital were selected,and 29 healthy people were selected as the control group.Early in the morning,3ml of fasting venous blood was collected.Renal carcinoma was pathologically confirmed.Whole blood 9-color,11 parameter flow cytometry was used to detect expression levels of MDSCs,Tregs,CD_(3)^(+)CD_(4)^(+)T cells,CD_(3)^(+)CD_(8)^(+)T cells,CD_(3)^(+)CD_(4)^(+)/CD_(3)^(+)CD_(8)^(+)T cells and CD_(3)^(+)CD16+CD56+T cells.Clinical data of patients were collected,including age,sex,body mass index(BMI),tumor staging,tumor size and pathology type.Results There were no significant differences in the sex,age and BMI between renal carcinoma patients and healthy volunteers in the results of flow cytometry(P>0.05).Significant differences were detected in the peripheral CD_(3)^(+)CD_(4)^(+)T cells,CD_(3)^(+)CD_(8)^(+)T cells,CD_(3)^(+)CD_(4)^(+)/CD_(3)^(+)CD_(8)^(+)T cells,natural killer(NK)cells,Tregs,polymorphonuclear MDSCs(PMN-MDSCs)and monocytic MDSCs(M-MDSCs)between groups(P<0.05).Specifically,renal carcinoma patients had significantly lower levels of CD_(3)^(+)CD_(4)^(+)T cells and CD_(3)^(+)CD_(8)^(+)T cells,but higher levels of Tregs,PMN-MDSCs and M-MDSCs than those of healthy volunteers(P<0.05).Peripheral CD_(3)^(+)CD_(4)^(+)T cells,CD_(3)^(+)CD_(8)^(+)T cells,CD_(3)^(+)CD_(4)^(+)/CD_(3)^(+)CD_(8)^(+)T cells,NK cells,Tregs,PMN-MDSCs and M-MDSCs in renal carcinoma patients did not correlate with the sex,age,BMI and pathological type(P>0.05).CD_(3)^(+)CD_(8)^(+)T cells,CD_(3)^(+)CD_(4)^(+)/CD_(3)^(+)CD_(8)^(+)T cells and NK cells did not correlate with tumor size and stagin

关 键 词：肾癌 骨髓源性抑制细胞 调节性T细胞 CD_(3)^(+)CD_(4)^(+)T细胞 CD_(3)^(+)CD_(8)^(+)T细胞 CD3-CD_(1)^(+)6CD_(5)^(+)6细胞 

分 类 号：R737.11[医药卫生—肿瘤]