凉粉草多糖提取和分级及抗氧化性能  

Extraction and Fraction of Polysaccharides from Mesona Chinensis Benth and Their Antioxygenic Property

作　　者：王治明 谢慧红[1] 邱顺健 游巧 袁滢 梁小兰 刘红霞[1] Zhiming Wang;Huihong Xie;Shunjian Qiu;Qiao You;Ying Yuan;Xiaolan Liang;Hongxia Liu(School of Material Science and Engineering,Guilin University of Technology,Guilin 541000,China)

机构地区：[1]桂林理工大学材料科学与工程学院,广西桂林541000

出　　处：《高分子材料科学与工程》2024年第6期99-105,共7页Polymer Materials Science & Engineering

基　　金：广西自然科学基金资助项目(2023GXNSFAA026140);广西科技基地和人才专项(2022AC18011);国家自然科学基金资助项目(52263030)。

摘　　要：文中采用热碱提取法结合超声技术制备了凉粉草多糖(MCP),然后进一步根据相似相溶的原理对凉粉草多糖进行分级,成功获得了分级多糖MCP-1和MCP-2,并通过单因素实验研究了超声时间和提取次数对凉粉草多糖提取率的影响。结果表明,凉粉草多糖的最佳提取条件为在超声功率400W时超声60min,碱提取4次,在此条件下,多糖产率为44.02%±0.06%。紫外和红外光谱测试表明MCP是一种含糖醛酸的粗多糖,凝胶渗透色谱测试其重均分子量(Mw)为4.991×105。而体外抗氧化实验表明,与MCP相比,MCP-1对羟基自由基的清除能力较强,MCP-2对超氧阴离子自由基的清除能力较强,在生物医药领域具有广阔的应用前景。Hot alkali extraction method combined with ultrasound technology was used to extract Mesona chinensis polysaccharide(MCP),then further graded polysaccharides MCP-1 and MCP-2 were also successfully prepared according to the principle of similar phase solubility.The effect of ultrasound time and extraction times on the extraction rate of Mesona chinensis polysaccharide were investigated by single-factor experiment.The results show that the optimal conditions for extracting MCP are 60 min of ultrasound time at 400 W and 4 times of alkali extraction,and the final yield of MCP is 44.02%±0.06%.MCP is characterized a kind of crude polysaccharide containing glyoxylate by UV and IR spectroscopy,and its average molecular weight(Mw)is 4.991×105 by gel permeation chromatography.In vitro antioxidant results show that compared with MCP,MCP-1 has stronger hydroxyl radical scavenging ability and MCP-2 has stronger superoxide anion radical scavenging ability,showing widely potential application in biomedical field.

关 键 词：凉粉草多糖 热碱提取法 超声 分级 自由基清除 

分 类 号：R284.2[医药卫生—中药学]