白藜芦醇通过介导GAL-3表达促进卵巢癌细胞凋亡并增强其化疗感性  

Resveratrol promotes ovarian cancer cell apoptosis and enhances its chemotherapy sensitivity by mediating GAL⁃3 expression

作　　者：李雅楠 王慧[1] 王静[1] 贺英[1] 马春星 陈丽华[1] 王思思[1] 焦桂清[1] 亢延飞 WANG Hui;WANG Jing;HE Ying;MA Chun-xing;CHEN Li-hua;WANGg Si-si;JIAO Gui-qing;KANG Yan-fei(Department of Gynecology,The First Affiliated Hospital of Hebei North University,Zhangjiakou,Hebei Province 075000,China)

机构地区：[1]河北北方学院附属第一医院妇科,河北张家口075000

出　　处：《解剖学研究》2024年第4期354-360,共7页Anatomy Research

基　　金：河北省医学科学研究课题(20200198)。

摘　　要：目的 探讨白藜芦醇(RES)对卵巢癌细胞凋亡和化疗敏感性的影响及其作用机制。方法研究样本为卵巢癌SKOV3细胞,分为3组,每组11株,均进行常规培养,对照组不给予干预,顺铂组给予10μmol/L顺铂干预,RES+顺铂组给予50μmol/L RES+10μmol/L顺铂干预。采用CCK-8实验检测细胞活力,BrdU实验检测细胞增殖,Annexin V-FITC/PI双染细胞凋亡试验检测细胞凋亡,比色测定试验检测caspase-3活性,Transwell细胞迁移实验检测细胞迁移能力,侵袭试验检测细胞侵袭能力,蛋白质印迹分析caspase-3、GAL-3、p-Akt、AKT、Iκβα、P65、Bcl-2、 p-IKKα/β蛋白相对表达。结果 RES+顺铂组、顺铂组、对照组细胞活力分别为(62±14)%、(74±16)%、(98±16)%,细胞增殖率分别为(59±16)%、(76±17)%、(97±17)%,RES+顺铂组、顺铂组细胞活力、细胞增殖率显著低于对照组,RES+顺铂组显著低于顺铂组(P<0.05);RES+顺铂组、顺铂组、对照组细胞凋亡率分别为(64.29±13.14)%、(47.48±9.52)%、(14.72±3.03)%,RES+顺铂组、顺铂组细胞凋亡率显著高于对照组,RES+顺铂组显著高于顺铂组(P<0.05);RES+顺铂组caspase-3活性、蛋白相对表达显著高于顺铂组、对照组(P<0.05);RES+顺铂组、顺铂组细胞迁移量、细胞侵袭量显著低于对照组,RES+顺铂组显著低于顺铂组(P<0.05);RES+顺铂组GAL-3、p-Akt蛋白相对表达显著低于顺铂组、对照组(P<0.05),各组AKT蛋白表达差异无统计学意义(P<0.05);RES+顺铂组、顺铂组p-IKKα/β、P65、Bcl-2、蛋白相对表达显著低于对照组,RES+顺铂组显著低于顺铂组(P<0.05);RES+顺铂组、顺铂组Iκβα蛋白相对表达显著高于对照组,RES+顺铂组显著高于顺铂组(P<0.05)。结论 RES诱导癌细胞凋亡可能与GAL-3水平降低有关。其作用机制可能是通过调节卵巢癌细胞凋亡、转移相关蛋白表达抑制其增殖、侵袭并增强其对顺铂的敏感性。Objective To investigate the effects of resveratrol(RES)on apoptosis and chemotherapy sensi-tivity of ovarian cancer cells and its mechanism.Methods The samples were ovarian cancer resistant cell line SKOV3 cell lines,which were divided into 3 groups with 11 cells in each group.The control group was routinely cul-tured,the cisplatin group was given 10μmol/L cisplatin intervention,and the RES+cisplatin group was givenμmol/L 50μmol/L RES+10μmol/L cisplatin intervention.Cell viability was detected by CCK-8 assay,cell proliferation was detected by BrdU assay,apoptosis was detected by Annexin V-FITC/PI double staining apoptosis assay,caspase-3 activity was detected by colorimetric assay,cell migration ability was detected by Transwell cell migration assay,and cell invasion assay was detected by invasion assay.Western blot analysis of caspase-3,GAL-3,p-Akt,AKT,Iκβα,P65,Bcl-2,p-IKKα/βprotein relative expression.Results The cell viability and cell proliferation rate in RES+cisplatin group and cisplatin group were significantly lower than those in control group,and the rate in RES+cisplatin group was significantly lower than that in cisplatin group(62%±14%,74%±16%,and 98%±16%;59%±16%,76%±17%and 97%±17%,respectively).The apoptosis rate of RES+cisplatin group and cisplatin group was significantly higher than that of control group,and that of RES+cisplatin group was significantly higher than that of cisplatin group(64.29%±13.14%,47.48%±9.52%,14.72%±3.03%,respectively)(P<0.05).The activity and relative expression of caspase-3 in RES+cisplatin group were significantly higher than those in cisplatin group and control group(P<0.05).The amount of cell migration and cell invasion in RES+cisplatin group and cisplatin group were significantly lower than those in control group,and RES+cisplatin group was significantly lower than that in cis-platin group(P<0.05);The relative expressions of GAL-3 and p-Akt protein in RES+cisplatin group were signifi-cantly lower than those in cisplatin group and control group(P<0.05).

关 键 词：卵巢癌 白藜芦醇 半乳糖凝集素-3 细胞凋亡 化疗敏感性 SKOV3细胞株 

分 类 号：R737.31[医药卫生—肿瘤]