基于BDNF/CREB信号通路探讨藏药十一味维命胶囊的抗抑郁作用机制  

Study on the Anti-depression Mechanism of Tibetan medicine Shi-Yi-Wei-Wei-Ming capsule Based on BDNF/CREB Signaling Pathway

作　　者：李鑫 王琼莹 马召田[1] 于雪[1] 孙洪昊 仁青东主[3] 久美彭措 东知加 任小巧[1,2] LI Xin;WANG Qiong-ying;MA Zhao-tian;YU Xue;SUN Hong-hao;Renqing Dong-zhu;Jiumei Peng-cuo;Dongzhijia;REN Xiao-qiao(Beijing University of Chinese Medicine,Beijing 100029,China;Institute of Ethnic Medicine,Beijing University of Chinese Medicine,Beijing 100029,China;Qinghai University,Xining 810016,China;Qinghai Jiumei Tibetan Hospital Co.,LTD.,Xining 810000,China)

机构地区：[1]北京中医药大学,北京100029 [2]北京中医药大学民族医药学研究所,北京100029 [3]青海大学,青海西宁810016 [4]青海久美藏医院有限公司,青海西宁810000

出　　处：《云南中医中药杂志》2024年第8期61-67,共7页Yunnan Journal of Traditional Chinese Medicine and Materia Medica

基　　金：青海省重点研发与转化计划项目(2021-SF-C21)。

摘　　要：目的基于BDNF/CREB信号通路对藏药十一味维命胶囊对抑郁症小鼠的抗抑郁作用及机制进行研究。方法将48只小鼠随机分为正常组(n=12)、造模组(n=36),造模组采用CUMS结合CRS方法造模3周。成模后,将造模组小鼠随机分为模型组、十一味维命胶囊组(藏药组)、氟西汀组,各12只,干预4周。采用行为学实验对抑郁症小鼠模型进行评估。WB法检测小鼠海马BDNF、GDNF、NGF、p-CREB/CREB、PSD-95表达;IHC法检测小鼠海马BDNF、GDNF、p-CREB MOD值。结果造模3周后,造模组小鼠出现抑郁样行为,小鼠体质量、糖水偏好率均低于正常组(P<0.01);不动时间长于正常组(P<0.01);给药4周后,模型组小鼠体质量、糖水偏好率低于正常组(P<0.01);不动时间长于正常组(P<0.01);藏药组、氟西汀组小鼠体质量、糖水偏好率均高于模型组(P<0.05或P<0.01);不动时间均短于模型组(P<0.01)。模型组小鼠海马BDNF、GDNF、PSD-95蛋白表达及BDNF、GDNF、p-CREB MOD值均低于正常组(P<0.05或P<0.01)。藏药组、氟西汀组小鼠海马BDNF、GDNF、p-CREB/CREB、PSD-95蛋白表达均高于模型组(P<0.05或P<0.01)。藏药组小鼠BDNF、GDNF MOD值均高于模型组(P<0.05或P<0.01);氟西汀组小鼠海马BDNF、GDNF、p-CREB MOD值均高于模型组(P<0.01)。结论十一味维命胶囊可以改善抑郁症小鼠的抑郁行为,其作用机制可能与调控BDNF/CREB信号通路有关。Objective:To study on the anti-depression effect and mechanism of Tibetan medicine Shi-Yi-Wei-Wei-Ming capsule on depressed mice based on BDNF/CREB signaling pathway.Methods:48 mice were randomly divided into normal group(n=12)and model group(n=36).CUMS combined with CRS method was used to make the model group for 3 weeks.After modeling,the mice were randomly divided into model group,Shi-Yi-Wei-Wei-Ming capsule group(Tibetan medicine group)and fluoxetine group,12 mice each group,and the intervention lasted for 4 weeks.Behavioral experiments were used to evaluate the depression mouse model.The expression of BDNF,GDNF,NGF,p-CREB/CREB and PSD-95 in hippocampus of mice were detected by WB method.The MOD values of BDNF,GDNF and p-CREB were determined by IHC.Results:After 3 weeks of modeling,the mice in the model group showed depression-like behavior,and the mice body mass and sucrose preference rate were lower than those in the normal group(P<0.01).The immobility time was longer than that of the normal group(P<0.01).After 4 weeks of administration,the body mass and sucrose preference rate of mice in the model group were lower than those in the normal group(P<0.01).The immobility time was longer than that of the normal group(P<0.01).The body mass and sucrose preference rate of mice in the Tibetan medicine group and fluoxetine group were higher than those in the model group(P<0.05 or P<0.01).The immobility time was shorter than that of the model group(P<0.01).The expressions of BDNF,GDNF,PSD-95 protein and the MOD values of BDNF,GDNF and P-CREB in the hippocampus of mice in the model group were lower than those in the normal group(P<0.05 or P<0.01).The protein expressions of BDNF,GDNF,P-CREB/CREB and PSD-95 in the hippocampus of mice in the Tibetan medicine group and the fluoxetine group were higher than those in model group(P<0.05 or P<0.01).The MOD values of BDNF and GDNF in the Tibetan medicine group were higher than those in the model group(P<0.05 or P<0.01).The hippocampal BDNF,GDNF and P-CREB MOD values of the flu

关 键 词：抑郁症 十一味维命胶囊 BDNF/CREB信号通路 小鼠 

分 类 号：R749.41[医药卫生—神经病学与精神病学]