外周血HMGB1Beclin1表达对多发性硬化急性发作期的诊断价值  

Diagnostic value of HMGB1 and Beclin1 expressions in peripheral blood in acute relapses of multiple sclerosis

作　　者：杨芳[1] 朱海生[1] YANG Fang;ZHU Haisheng(Handan Central Hospital,Handan 057150,China)

机构地区：[1]邯郸市中心医院,河北邯郸057150

出　　处：《中国实用神经疾病杂志》2024年第9期1136-1140,共5页Chinese Journal of Practical Nervous Diseases

基　　金：河北省卫生计生委项目(编号:20181687)。

摘　　要：目的探究高迁移率族蛋白B1(HMGB1)和Beclin1表达对急性发作期多发性硬化(MS)的诊断价值,分析其表达与病情严重程度的相关性。方法回顾性选取2020-01—2022-12邯郸市中心医院收治的86例急性发作期MS患者为研究组,根据Kurtzke扩展残疾状态评估量表(EDSS)对患者病情进行评估,选取同期健康体检者95例为对照组,收集2组患者一般资料,采集外周血以酶联免疫吸附法进行HMGB1和Beclin1检测,采用受试者工作特征(ROC)曲线分析HMGB1和Beclin1表达对急性发作期MS的诊断价值,同时采用Pearson相关性分析其表达水平与疾病严重程度的关系。结果2组患者性别、年龄、体重指数(BMI)、淋巴细胞和嗜酸性粒细胞数量差异均无统计学意义(P>0.05),研究组患者白细胞和中性粒细胞均多于对照组(P<0.05)。研究组患者外周血中HMGB1表达水平高于对照组,Beclin1表达水平低于对照组(P<0.05)。经ROC曲线分析,HMGB1和Beclin1诊断急性发作期MS的AUC分别为0.798和0.809,两者联合预测效能最高,灵敏度和特异度分别为78.7%、93.7%。研究组患者EDSS评分为(3.38±1.02)分,随着EDSS评分的上升,HMGB1表达水平上升,Beclin1表达水平下降。经Pearson相关分析,急性发作期MS患者EDSS评分与HMGB1表达呈正相关,与Beclin1表达呈负相关(P<0.05)。结论HMGB1和Beclin1表达对急性发作期MS具有一定的诊断价值,两者联合诊断效能最佳,患者病情严重程度与HMGB1表达水平呈正相关,与Beclin1呈负相关。Objective To evaluate the diagnostic value of high-mobility group protein B1(HMGB1)and Beclin1 expressions in peripheral blood in acute relapses of multiple sclerosis(MS),and analyze their relationships with the severity of the disease.Methods Eighty-six patients with acute relapses of MS admitted to Handan Central Hospital from January 2020 to December 2022 were retrospectively selected as study group,and were assessed using Kurtzke extended disability status scale(EDSS).In the same period,95 healthy subjects were set as control group.General data were collected from patients in both groups,and peripheral blood samples were collected for HMGB1 and Beclin1 expressions detection by enzyme-linked immunosorbent assay.The receiver operating characteristic(ROC)curve was plotted to evaluate the value of HMGB1 and Beclin1 expressions in peripheral blood in the diagnosis of acute relapses of MS,and Pearson correlation analysis was conducted to discuss the correlation of two indicators with the severity of disease.Results The gender,age,body mass index(BMI),number of lymphocytes and eosinophils yielded no statistical difference between two groups(P>0.05),but the numbers of peripheral blood leukocytes and neutrophils were larger in study group than those in control group(P<0.05).Study group demonstrated higher HMGB1 expression in peripheral blood compared to control group expression and lower Beclin1(P<0.05).ROC curve analysis denoted that the area under the curve(AUC)of HMGB1 and Beclin1 in the diagnosis of acute relapses of MS were 0.798 and 0.809,respectively,with the sensitivity and specificity of and the combined detection of the two obtained better predictive efficacy than the separate test,78.7%and 93.7%,respectively.The mean EDSS score of study group was(3.38±1.02)points.With the increase of EDSS score,there was an increasing trend in HMGB1 expression and a decreasing trend in Beclin correlated with HMGB 1 expression.Pearson correlation analysis indicated that EDSS score was positively 1 expression and negatively

关 键 词：多发性硬化 急性发作期 Kurtzke扩展残疾状态评估量表 高迁移率族蛋白B1 BECLIN1 

分 类 号：R744.51[医药卫生—神经病学与精神病学]