激光共聚焦显微镜荧光漂白后恢复实验的制样优化  

Optimization of Sample Preparation for FRAP by Laser Scanning Confocal Microscopy

作　　者：赵新英 刘菲[2] 徐萌 Zhao Xinying;Liu Fei;Xu Meng(College of Life Science,Shandong Agricultural University,Tai'an,271018,China;College of Life Science,Nankai University,Tianjin,300071,China)

机构地区：[1]山东农业大学生命科学院,山东泰安271018 [2]南开大学生命科学院,天津300071

出　　处：《中国现代教育装备》2024年第15期25-28,共4页China Modern Educational Equipment

基　　金：山东省研究生优质教育教学资源项目(编号:SDYKC2022033);山东农业大学研究生教育教学改革研究项目(编号:2022KC001)。

摘　　要：利用激光共聚焦显微镜进行荧光漂白后恢复(FRAP)实验为研究分子的流动性提供了新的手段。然而,在拟南芥根毛细胞中进行荧光漂白后恢复实验时,由于拍摄时长的影响经常会导致样品脱离原来的焦平面,从而无法获得可靠的数据。以拟南芥根尖为材料,通过实验比较激光共聚焦显微镜下的常规制片、指甲油封片以及甘油制片,结果表明,利用指甲油封片能更好地解决样品离焦问题,可以获取有效的实验数据。The fluorescence recovery after photobleaching(FRAP)by laser scanning confocal microscopy provides a new way to study the fluidity of molecules.However,in the recovery experiment of Arabidopsis root hairs after photobleaching,the sample often deviates from the original focal plane due to the loss of time and in turn the reliabile data cannot be obtained.In this study,by comparing the conventional sample preparation,glycerol preparation and sealing the cover glass by nail polish,the results show that the defocusing problem of the sample can be better resolved by the nail polish,which is helpful to obtain the effective experimental data.

关 键 词：激光共聚焦 荧光漂白后恢复 拟南芥 根尖 

分 类 号：Q-33[生物学] G434[文化科学—教育学]