石灰土易提取球囊霉素相关土壤蛋白的实验条件优化  

Optimization of Extraction Method for Easily Extractable Glomalin-Related Soil Protein from Calcareous Soil

作　　者：蒋利 康志强 梁月明[2] 吴泽燕[2] 涂纯 李强[2] JIANG Li;KANG Zhiqiang;LIANG Yueming;WU Zeyan;TU Chun;LI Qiang(Environmental Science and Engineering College,Guilin University of Technology,Guilin 541004,China;Key Laboratory of Karst Dynamics,Ministry of Natural Resources and Guangxi,Institute of Karst Geology,Chinese Academy of Geological Sciences,Guilin 541004,China;Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area,Guilin University of Technology,Guilin 541006,China;Bureau of Geology and Mineral Prospecting and Exploitation of Guangxi Zhuang Autonomous Region,Nanning 530023,China)

机构地区：[1]桂林理工大学环境科学与工程学院,广西桂林541006 [2]中国地质科学院岩溶地质研究所,自然资源部/广西岩溶动力学重点实验室,广西桂林541004 [3]广西岩溶地区水污染控制与用水安全保障协同创新中心,广西桂林541006 [4]广西壮族自治区地质矿产勘查开发局,广西南宁530023

出　　处：《岩矿测试》2024年第4期582-591,共10页Rock and Mineral Analysis

基　　金：国家自然科学基金项目(42172341);广西重点研发计划项目(桂科AB21196050);中国地质科学院岩溶地质研究所基本科研业务费项目(2023020)。

摘　　要：在水-二氧化碳-碳酸盐岩-生物的相互作用下,全球形成8.24×10^(8)t C/a的岩溶碳汇,其中部分岩溶碳汇以土壤有机质的形式固存,进而在国家双碳目标中发挥重要作用。全球分布广泛的丛枝菌根真菌分泌的球囊霉素相关土壤蛋白(Glomalin-related soil protein,GRSP)性质稳定、不易分解,是土壤有机质的重要组成部分。提取合格的GRSP是深入研究岩溶土壤有机碳汇的基础,然而以往研究因GRSP提取量不高、提取不充分、产物不专一等问题,以至其在有机质中发挥的作用机制无法深入开展研究,因此,提高GRSP提取量对于探究岩溶土壤有机质的形成和稳定机制具有重要意义。本文选取岩溶区黑色、棕色、黄色和红色四种石灰土,通过温度和时间正交实验,筛选出适用于岩溶土壤颗粒态有机质(POM)和矿物结合态有机质(MAOM)易提取球囊霉素相关土壤蛋白(EE-GRSP)的最佳提取条件。实验结果表明,POM和MAOM在123℃和80min条件下提取时,EE-GRSP提取量最高。应用于四类石灰土后,EE-GRSP增量范围为4.6%~34.2%。相较于全土,MAOM具有更高的稳定性与更强的有机碳保护能力。因此,随着温度和提取时间的提高,MAOM中的EE-GRSP得到更完全的释放,提取量显著提高。由此可见,EE-GRSP提取条件优化对深入研究岩溶土壤碳汇潜力及其稳定机制具有重要意义。Glomalin-related soil protein(GRSP)secreted by arbuscular mycorrhizal fungi is widely distributed worldwide,has stable properties and is not easily decomposed,which is an important component of soil organic matter.Extracting high quality GRSP is important for in-depth research on organic carbon sinks in calcareous soil from karst areas.However,in previous studies,the mechanism of GRSP in organic matter could not be further studied due to low extraction yield,insufficient extraction and non-specific products.Therefore,the high extraction amount of GRSP is of great significance to explore the formation and stabilization mechanism of organic matter in calcareous soil.This experiment selected four types of calcareous soil from karst areas:black,brown,yellow and red calcareous soil.By using orthogonal experiments of temperature and time,the optimal extraction conditions for easily extractable glomalin-related soil protein(EE-GRSP)related to particulate organic matter(POM)and mineralassociated organic matter(MAOM)in calcareous soil were selected.The experimental results showed that the highest extraction amount of EE-GRSP was achieved when POM and MAOM were extracted at 123℃and 80min.After application into the four types of calcareous soil,the EE-GRSP contents increased from 4.6% to 34.2%.

关 键 词：易提取球囊霉素相关土壤蛋白 提取条件优化 颗粒态有机质 矿物结合态有机质 

分 类 号：S151.9[农业科学—土壤学]