人体胆汁中微塑料的检测与分析  

Detection and Analysis of Microplastics in Human Bile

作　　者：李小康 胡乐 黄冬冬 晏华军 胡勇军 张涛[4] 胡少辉[4] 殷强 朱泉[5] 刘汉忠 吴尚 钟碧波[7] 袁岸龙[7] Li Xiaokang;Hu Le;Huang Dongdong;Yan Huajun;Hu Yongjun;Zhang Tao;Hu Shaohui;Yin Qiang;Zhu Quan;Liu Hanzhong;Wu Shang;Zhong Bibo;Yuan Anlong(Postgraduate Training Base,Xiaogan Central Hospital,Wuhan University of Science and Technology,Xiaogan 432100,China;School of Medicine,Department of Medicine,Wuhan University of Science and Technology,Wuhan 430081,China;Graduate Training Base of Jinzhou Medical University(Xiaogan Hospital Affiliated to Wuhan University of Science and Technology),Xiaogan 432100,China;Department of Hepatobiliary Surgery,Xiaogan Hospital Affiliated to Wuhan University of Science and Technology,Xiaogan 432100,China;Operation Room,Xiaogan Hospital Affiliated to Wuhan University of Science and Technology,Xiaogan 432100,China;Department of Pathology,Xiaogan Hospital Affiliated to Wuhan University of Science and Technology,Xiaogan 432100,China;Department of Gastroenterology,Xiaogan Hospital Affiliated to Wuhan University of Science and Technology,Xiaogan 432100,China)

机构地区：[1]武汉科技大学孝感市中心医院研究生培养基地,孝感432100 [2]武汉科技大学医学部医学院,武汉430081 [3]锦州医科大学研究生培养基地(武汉科技大学附属孝感医院),孝感432100 [4]武汉科技大学附属孝感医院肝胆外科,孝感432100 [5]武汉科技大学附属孝感医院手术室,孝感432100 [6]武汉科技大学附属孝感医院病理科,孝感432100 [7]武汉科技大学附属孝感医院消化内科,孝感432100

出　　处：《生态毒理学报》2024年第4期311-323,共13页Asian Journal of Ecotoxicology

基　　金：湖北省卫生和计划生育委员会资助项目(WJ2018H0109);孝感市自然科学计划项目(XGKJ2021010069)。

摘　　要：微塑料(MPs)因其广泛分布及其对自然生态系统的影响而受到越来越多关注,但迄今为止还没有研究报告微塑料在人体胆汁中的暴露,本研究通过对人体胆汁中的微塑料进行定性和定量分析,以期为今后深入探究微塑料与人类疾病的关系提供依据。纳入武汉科技大学附属孝感医院2023年3月—5月确诊为胆囊结石并行腹腔镜下胆囊切除术患者12例,收集患者的胆汁样本作为实验组,以程序空白、实验空白及仪器空白作为对照组,采用热解-气相色谱/质谱(Py-GC/MS)方法检测2组样本中微塑料的种类及含量。在12个胆汁样本中检测到聚氯乙烯(PVC)、聚对苯二甲酸乙二醇酯(PET)、尼龙66(PA66)和聚乙烯(PE)共4种微塑料类型,在单个样本中可检测到1~3种不同的微塑料种类,检测结果阳性率为100%(12/12),其中PVC阳性率为100%(12/12),PA66阳性率为91.66%(11/12),PET阳性率为25.0%(3/12),PE阳性率为8.33%(1/12)。在检测到该塑料颗粒的样本中,PA66含量为(10.36±8.62)μg·g^(-1),PVC平均含量为14.53μg·g^(-1),PET平均含量为10.66μg·g^(-1),在唯一检测到PE的样本中,其含量为3.01μg·g^(-1)。本研究对人体胆汁中的微塑料进行定性和定量分析,为微塑料与人类疾病的深入探讨提供依据。Microplastics(MPs)have attracted increasing attention due to their wide distribution and impact on n atural ecosystems.However,there have been no reports on the exposure of microplastics in human bile to date.This study conducted qualitative and quantitative analysis of microplastics in human bile,in order to provide a basis for further exploration of the relationship between microplastics and human diseases.12 patients diagnosed with g allstones and underwent laparoscopic cholecystectomy in Xiaogan Hospital,which is affiliated to Wuhan University of Science and Technology,were included from March to May 2023.The bile samples from those patients were c ollected as the experimental group,while the procedure blanks,experimental blanks and instrument blanks were used as control groups.Pyrolysis-gas chromatography/mass spectrometry(Py-GC/MS)method was employed to detect the types and amounts of microplastics in the two groups of samples.Four types of microplastics,polyvinyl chloride(PVC),polyethylene terephthalate(PET),nylon 66(PA66),and polyethylene(PE),were detected in 12 bile samples.One to three different types of microplastics were detected in individual samples,and the positive rate was 100%(12/12).The positive rate of PVC,PA66,PET,and PE were 100%(12/12),91.66%(11/12),25.0%(3/12),and 8.33%(1/12),respectively.In the samples where the plastic particles were detected,the content of PA66 was(10.36±8.62)μg·g^(-1),the average content of PVC was 14.53μg·g^(-1),and the average content of PET was 10.66μg·g^(-1).In the sole sample where PE was detected,the content was 3.01μg·g^(-1).In this study,the qualitative and quantitative analysis of microplastics in human bile was conducted to provide a basis for the further exploration of relationship between microplastics and human diseases.

关 键 词：微塑料 人体 胆汁 热解-气相色谱/质谱 

分 类 号：X171.5[环境科学与工程—环境科学]