A 9.5-kb deletion in the 1st intron of OsMADS51 enhances temperature sensitivity in rice  

作　　者：Lei Zhao Haifei Hu Junyu Chen Chongrong Wang Yibo Chen Hong Li Daoqiang Huang Zhidong Wang Degui Zhou Rong Gong Yangyang Pan Junliang Zhao Liangyong Ma Shaochuan Zhou 

机构地区：[1]Rice Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,Guangdong,China [2]State Key Laboratory of Rice Biology and Chinese National Center for Rice Improvement,China National Rice Research Institute,Hangzhou 310006,Zhejiang,China [3]Guangdong Laboratory for Lingnan Modern Agriculture,Guangzhou 510640,Guangdong,China [4]Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China(Co-construction by Ministry and Province),Ministry of Agriculture and Rural Affairs,Guangzhou 510640,Guangdong,China [5]Guangdong Rice Engineering Laboratory,Guangzhou 510640,Guangdong,China [6]Guangdong Key Laboratory of New Technology in Rice Breeding,Guangzhou 510640,Guangdong,China

出　　处：《The Crop Journal》2024年第4期1031-1040,共10页作物学报（英文版）

基　　金：supported by the Laboratory of Lingnan Modern Agriculture Project(NT2021001);Guangdong Province Basic and Applied Basic Research Fund-Provincial and Municipal Joint Fund Project(2023A1515110882);Guangzhou Science and Technology Plan Project(2023A04J0811);Guangdong Province Rural Revitalization Strategy Special Fund Seed Industry Revitalization Project(2022-NPY-00-013);Natural Science Foundation of Zhejiang Pro-vince(LY22C130006);Key Laboratory of New Rice Breeding Technologies in Guangdong Province(2023B1212060042).

摘　　要：Temperature is an important environmental factor affecting heading date of rice.Despite its importance,genes responsible for temperature-sensitive heading in rice have remained elusive.Our previous study identified a quantitative trait locus qHd1 which advances heading date under high temperatures.A 9.5-kb insertion was found in the first intron of OsMADS51 in indica variety Zhenshan 97(ZS97).However,the function of this natural variant in controlling temperature sensitivity has not been verified.In this study,we used CRISPR/Cas9 to knock out the 9.5-kb insertion in ZS97.Experiments conducted under cotrolled conditions in phytotrons confirmed that deletion increased temperature sensitivity and advanced heading by downregulating the expression level of OsMADS51.One-hybrid assays in yeast,ChIP-quantitative polymerase chain reaction,electrophoretic mobility shift,and luciferase-based transient transactivation assays collectively confirmed that OsMADS51 affects heading date by regulation of heading date gene Ehd1.We further determined that the long non-coding RNA HEATINR is generated from the first intron of OsMADS51,offering an explanation for how the 9.5-kb insertion affects temperature sensitivity.We also found that OsMADS51 was strongly selected in early/late-season rice varieties in South China,possibly accounting for their strong temperature sensitivity.These insights not only advance our understanding of the molecular mechanisms underlying the temperature-responsive regulation of heading date in rice but also provide a valuable genetic target for molecular breeding.

关 键 词：OsMADS51 INSERTION Long non-coding RNA Oryza sativa 

分 类 号：S511[农业科学—作物学]