结直肠癌组织中DJ-1表达对患者区域淋巴结转移及预后的临床意义  

Clinical significance of DJ-1 expression in colorectal cancer tissues for regional lymph node metastasis and prognosis in patients

作　　者：段杨丽 冯洁 秦家丽 杨华 DUAN Yangli;FENG Jie;YANG Hua;QIN Jiali(Department of Gastroenterology,Guilin People’s Hospital,Guilin 541002,China;Department of Pathology,Guilin People’s Hospital,Guilin 541002,China)

机构地区：[1]桂林市人民医院消化内科,541002 [2]桂林市人民医院病理科,541002

出　　处：《国际消化病杂志》2024年第4期233-240,共8页International Journal of Digestive Diseases

基　　金：广西壮族自治区卫生健康委员会科技研究计划课题(Z20200725)。

摘　　要：目的 分析结直肠癌(CRC)组织中蛋白/核酸去糖酶1(DJ-1)表达与患者区域淋巴结转移(LNM)及预后的相关性。方法 选择2017年6月至2022年4月在桂林市人民医院接受根治性手术的258例CRC患者作为研究对象,收集了258例患者的CRC组织,其中81例患者有配对的癌旁组织。另选择同期经内镜下治疗的58例患者的结直肠腺瘤组织作为对照。分析CRC组织中不同DJ-1蛋白表达与患者临床病理特征的关系。采用免疫组织化学法分析组织中DJ-1表达情况。采用多因素logistic回归分析探讨区域LNM的预测因素。采用单因素和多因素Cox回归分析探讨影响CRC患者复发及死亡的因素。采用Kaplan-Meier生存曲线分析不同DJ-1表达患者的5年无复发生存率和生存率。结果 3组的DJ-1蛋白表达差异有统计学意义(χ^(2)=202.557,P<0.001)。CRC组织中DJ-1蛋白中度表达和强表达患者的占比均显著高于癌旁组织和结直肠腺瘤组织(P均<0.05)。高表达组患者的年龄偏大,55.56%患者的原发病灶位于左半结肠,低分化CRC患者的占比较高,患者的区域LNM阳性率也较高(P均<0.05)。多因素logistic回归分析结果显示,CRC组织中DJ-1蛋白高表达能独立预测区域LNM和低分化CRC(P均<0.05)。258例患者的5年无复发生存率和生存率分别67.44%和70.54%。单因素和多因素Cox回归分析结果显示,CRC组织中DJ-1蛋白高表达为CRC患者复发和死亡的独立危险因素(P均<0.05)。上述结果与基于Oncomine数据库和TCGA队列的生物信息学分析结果基本一致。结论 CRC组织中DJ-1蛋白高表达与区域LNM相关,这可能是患者预后的影响因素。DJ-1有潜力成为预测CRC患者区域LNM和预后的生物标志物。Objective This paper intends to analyze the correlation between protein/nucleic acid decarboxylase 1(DJ-1)expression in colorectal cancer(CRC)tissue and regional lymph node metastasis(LNM)as well as patient prognosis.Methods Two hundred and fifty-eight CRC patients who underwent radical surgery at Guilin People’s Hospital from June 2017 to April 2022 were selected as the research subjects.CRC tissues from the patients were collected,of which 81 patients had paired adjacent cancer tissues.Another 58 patients who underwent endoscopic treatment during the same period were selected as controls for colorectal adenoma tissue.The relationship between the expression of different DJ-1 proteins in CRC tissues and the clinical pathological characteristics of patients were analyzed.The immunohistochemistry was used to analyze the expression of DJ-1 in tissues.The multivariate logistic regression analysis was used to explore the predictive factors of regional LNM.The univariate and multivariate Cox regression analyses were used to explore the factors affecting the recurrence and death of CRC patients.The Kaplan-Meier survival curves were used to analyze the 5-year recurrence free survival rate and the cumulative survival rate of patients with different DJ-1 expressions.Results There is a statistically significant difference in the expression of DJ-1 protein among the three groups(χ2=202.557 and P<0.001).The proportion of patients with moderate and strong expression of DJ-1 protein in CRC tissues is significantly higher than that in adjacent cancer tissues and colorectal adenoma tissues(P<0.05).The age of patients in the high expression group is relatively older,with 55.56%of patients having primary lesions located in the left colon.The proportion of poorly differentiated CRC patients is relatively high,and the positive rate of regional LNM in patients is also high(P<0.05).The results of logistic regression multivariate analysis show that the high expression of DJ-1 protein in CRC tissues could independently predict regiona

关 键 词：蛋白/核酸去糖酶1 区域淋巴结转移 结直肠癌 预后 

分 类 号：R735.3[医药卫生—肿瘤]