SREBP2、LOXL2和PEBP4在原发性肝癌中的表达及与患者临床病理特征的相关性  

Expression of SREBP2,LOXL2,and PEBP4 in primary liver cancer and its correlation with clinical pathological characteristics of patients

作　　者：高文广 李龙辉 王建华 GAO Wenguang;WANG Jianhua;LI Longhui(Department of Oncology,the Fourth People’s Hospital of Langfang,Langfang 065700,China;Department of Gastroenterology,the Fourth People’s Hospital of Langfang,Langfang 065700,China)

机构地区：[1]廊坊市第四人民医院肿瘤科,065799 [2]廊坊市第四人民医院消化内科,065799

出　　处：《国际消化病杂志》2024年第4期251-257,共7页International Journal of Digestive Diseases

基　　金：廊坊市科学技术研究与发展计划项目(2020013058)。

摘　　要：目的 探讨胆固醇调节元件结合蛋白2(SREBP2)、赖氨酰氧化酶样蛋白2(LOXL2)和磷脂酰乙醇胺结合蛋白4(PEBP4)在原发性肝癌(以下简称肝癌)中的表达及与患者临床病理特征的相关性。方法 选择2016年1月至2019年12月在廊坊市第四人民医院接受手术治疗的72例原发性肝癌患者作为研究对象,收集其临床资料。采用实时荧光定量PCR法检测组织中SREBP2、LOXL2和PEBP4的mRNA表达水平,分析肝癌组织中SREBP2、LOXL2和PEBP4的mRNA表达与患者临床病理特征及血管生成拟态(VM)的相关性,并分析肝癌组织中不同SREBP2、LOXL2和PEBP4 mRNA表达的患者的生存情况。结果 肝癌组织中SREBP2、LOXL2和PEBP4的mRNA表达水平均较癌旁组织显著升高(P均<0.05)。肝癌组织中SREBP2、LOXL2和PEBP4的m RNA表达与患者的TNM分期、病理分级、脉管侵犯均有相关性(P均<0.05)。72例患者的肝癌组织中有27例(37.50%)VM形成,VM染色阳性的肝癌组织中SREBP2、LOXL2和PEBP4的mRNA表达水平均显著高于VM染色阴性的肝癌组织(P均<0.05)。多因素logistic回归分析结果显示肝癌组织中SREBP2、LOXL2和PEBP4的mRNA高表达均为VM的危险因素(P均<0.05)。Kaplan-Meier生存分析结果显示,肝癌组织中SREBP2、LOXL2和PEBP4 mRNA高表达者的3年总生存率均较低表达者显著降低(P均<0.05)。结论 肝癌组织中SREBP2、LOXL2和PEBP4的m RNA均呈高表达,且与患者的部分临床病理特征、肝癌细胞VM形成均相关,并可影响预后。Objective An attempt is made in this paper to explore the expression of cholesterol regulatory element binding protein 2(SREBP2),lysyl oxidase-like protein 2(LOXL2),and phosphatidylethanolamine binding protein 4(PEBP4)in primary liver cancer(referred to as liver cancer)and their correlation with clinical pathological characteristics.Methods Seventy-two patients with primary liver cancer who underwent surgical treatment at the Fourth People's Hospital of Langfang from January 2016 to December 2019 were selected as the research subjects,and their clinical data were collected.The real-time fluorescence quantitative PCR was used to detect the mRNA expression levels of SREBP2,LOXL2,and PEBP4 in liver cancer tissues.The correlation between the mRNA expression of SREBP2,LOXL2,and PEBP4 in liver cancer tissues and the clinical pathological characteristics and vascular mimicry(VM)of the patients was analyzed.The survival status of the patients with different SREBP2,LOXL2,and PEBP4 mRNA expressions in liver cancer tissues was also analyzed.Results The mRNA expression levels of SREBP2,LOXL2,and PEBP4 in liver cancer tissues were significantly higher than those in adjacent tissues(P<0.05).The mRNA expression of SREBP2,LOXL2,and PEBP4 in liver cancer tissues are correlated with the TNM staging,pathological grade,and vascular invasion of patients(P<0.05).Out of liver cancer tissues of the 72 patients,27 cases(37.50%)have VM formation.The mRNA expression levels of SREBP2,LOXL2,and PEBP4 in liver cancer tissues with VM staining positive are significantly higher than those in liver cancer tissues with VM staining negative(P<0.05).The analysis of multivariate logistic regression equation shows that high mRNA expression of SREBP2,LOXL2,and PEBP4 in liver cancer tissues are all risk factors for VM(P<0.05).The Kaplan-Meier survival analysis results show that the 3-year overall survival rates of patients with high expression of SREBP2,LOXL2,and PEBP4 mRNA in liver cancer tissues are significantly lower than those with low expression(P

关 键 词：胆固醇调节元件结合蛋白2 赖氨酰氧化酶样蛋白2 磷脂酰乙醇胺结合蛋白4 原发性肝癌 临床病理特征 血管生成拟态 

分 类 号：R735.7[医药卫生—肿瘤]