高效液相色谱法同时测定洗发水中10种防腐剂的含量  

Simultaneous Determination of Ten Preservatives in Shampoo by HPLC

作　　者：张铨 刘艳云 麦运佳 梁少东 伍国怡 ZHANG Quan;LIU Yanyun;MAI Yunjia;LIANG Shaodong;WU Guoyi(Beihai Public Inspection and Testing Center,Beihai 536000,Guangxi,China)

机构地区：[1]北海市公共检验检测中心,广西北海536000

出　　处：《香料香精化妆品》2024年第4期169-172,共4页Flavour Fragrance Cosmetics

摘　　要：建立高效液相色谱法(HPLC)同时测定洗发水中苯酚等10种防腐剂含量的方法。样品经甲醇提取后,使用Capcellpak C_(18) MGⅢ柱(250 mm×4.6 mm×5μm)色谱柱,以乙腈、甲醇、0.02 mol/L乙酸铵溶液为流动相,梯度洗脱。采用二极管阵列检测器在280 nm波长下同时测定洗发水中10种防腐剂。通过试验探究,在质量浓度5~100μg/mL,10种防腐剂质量浓度与峰面积呈良好的线性关系(r~2≥0.998);检出限(LOD)为0.4~2.2μg/g,定量限(LOQ)为1.2~7.3μg/g,样品平均加标回收率为85.2%~107.4%,相对标准偏差(RSD)为0.7%~4.8%(n=6)。结果表明该方法操作简单,灵敏度高,结果准确,重复性好,可用于洗发水中多种防腐剂的同时测定。A method for simultaneous determination of 10 preservatives such as phenol in shampoo by high performance liquid chromatography(HPLC)was established.After methanol extraction,the samples were eluted by gradient elution on a Capcellpak C18 MGⅢcolumn(250 mm×4.6 mm×5μm)with acetonitrile,methanol and 0.02 mol/L ammonium acetate solution as mobile phases.A diode array detector was used to detect 10 preservatives in shampoo at 280 nm.In the range of 5-100μg/mL,the mass concentration of 10 preservatives had a good linear relationship with the peak area(r2≥0.998).The limits of detection(LOD)were 0.4-2.2μg/g,the limits of quantification(LOQ)were 1.2-7.3μg/g,the average recoveries were 85.2%-107.4%,and the relative standard deviation(RSD)was 0.7%-4.8%(n=6).The results showed that the method was simple,sensitive,accurate and repeatable,and could be used for simultaneous determination of various preservatives in shampoo.

关 键 词：高效液相色谱法 洗发水 防腐剂 

分 类 号：TQ658.3[化学工程—精细化工] O657.72[理学—分析化学]