非小细胞肺癌术后自身抗体水平及临床意义  

Postoperative autoantibody levels and clinical significance in non-small cell lung cancer

作　　者：王泽仪 穆银玉[1] 谢服役[1] WANG Zeyi;MU Yinyu;Xie Fuyi(Laboratory Department,Ningbo Medical Center,Li Huili Hospital,Ningbo 315040,Zhejiang,China)

机构地区：[1]宁波市医疗中心李惠利医院检验科,浙江宁波315040

出　　处：《中国现代医生》2024年第23期16-20,共5页China Modern Doctor

基　　金：浙江省医药卫生科技计划项目(2023KY246,2019KY606)。

摘　　要：目的探讨非小细胞肺癌(non-small cell lung cancer,NSCLC)患者手术前后肿瘤相关7项自身抗体(tumor-associated autoantibody,TAAB),包括肿瘤蛋白53(tumor protein 53,p53)、蛋白基因产物9.5(protein gene product9.5,PGP9.5)、性别决定基因家族2(SRY-box containing gene 2,SOX2)、G抗原7(G antigen 7 ATP-dependent RNA helicase,GAGE7)、ATP结合RNA解螺旋酶(ATP-dependent RNA helicase,GBU4-5)、人黑色素瘤抗原A1(melanoma antigen A1,MAGEA1)、肿瘤/睾丸抗原(cancer/testisantigen,CAGE)表达水平动态变化的临床意义。方法选取2019年1月至2022年8月在宁波市医疗中心李惠利医院胸外科接受手术治疗的NSCLC患者41例,采用酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)检测患者血清7项TAAB表达水平。结果鳞癌组PGP9.5手术前后表达水平均明显高于腺癌组(P<0.05),腺癌组GAGE7手术前后表达水平均明显高于鳞癌组(P<0.05)。鳞癌组术后1周GBU4-5表达水平明显高于腺癌组(Z=-2.095,P=0.036)。腺癌组内,术后1周GBU4-5(P=0.027)、CAGE(P=0.042)表达水平较术前明显下降。术后6个月PGP9.5(P=0.004)、GAGE7(P<0.001)、GBU4-5(P=0.014)表达水平较术前明显下降。鳞癌组中,术后1周表达水平差异均无统计学意义(P>0.05)。术后6个月SOX2(P=0.011)表达水平较术前明显下降。与术前比较,术后1周GBU4-5表达水平明显下降(P=0.009)。术后6个月PGP9.5(P=0.003)、GAGE7(P=0.001)、GBU4-5(P=0.013)表达水平明显下降。结论手术干预可降低NSCLC患者7项TAAB表达水平,手术治疗前后监测7项TAAB有助于NSCLC患者手术疗效的评估和术后病情进展的预测。Objective To investigate the clinical significance of dynamic changes in the expression levels of tumor associated autoantibody(TAAB),including tumor protein 53(p53),protein gene product 9.5(PGP9.5),SRY-box containing gene 2(SOX2),G antigen 7 ATP-dependent RNA helicase(GAGE7),ATP-dependent RNA helicase(GBU4-5),melanoma antigen A1(MAGE A1),cancer/testis antigen(CAGE)in non-small cell lung cancer(NSCLC)patients before and after surgery.Methods Forty-one patients with NSCLC who underwent surgical treatment at the Department of Thoracic Surgery of Li Huili Hospital,Ningbo Medical Center,were selected from January 2019 to August 2022.Enzyme linked immunosorbent assay(ELISA)was used to detect the expression levels of seven TAAB markers in the serum of 41 NSCLC patients undergoing surgical treatment.Results The expression levels of PGP9.5 were found to be significantly higher in the squamous cancer group than in the adenocarcinoma group(P<0.05),while the expression levels of GAGE7 were found to be significantly higher in the adenocarcinoma group than in the squamous cancer group(P<0.05).The expression level of GBU4-5 was found to be significantly higher in the squamous carcinoma group than in the adenocarcinoma group at one week after surgery(Z=-2.095,P=0.036).Within the adenocarcinoma group,the expression levels of GBU4-5(P=0.027)and CAGE(P=0.042)were found to be significantly lower at one week after surgery compared with those before surgery.The expression levels of PGP9.5(P=0.004),GAGE7(P<0.001),and GBU4-5(P=0.014)were found to have decreased significantly at six months postoperatively in comparison to their preoperative levels.In the squamous cancer group,no statistically significant differences were observed in the expression levels at one week after surgery.The expression level of SOX2(P=0.011)exhibited a statistically significant decrease at six months postoperatively in comparison to the preoperative levels.Similarly,the expression level of GBU4-5 was found to be significantly decreased at one week postoperative

关 键 词：非小细胞肺癌 肿瘤相关自身抗体 肺切除术 预测 

分 类 号：R734.2[医药卫生—肿瘤]