骆驼刺可培养内生细菌的分离及功能特性  

Isolation and functional characterization of culturable endophytic bacteria from Alhagi sparsifolia Shap.

作　　者：木尼热·木塔力甫 叶艺洁 张芯语 艾尼江·尔斯满 张志东[1] 王伟 楚敏[1] Munire Mutalifu;YE Yijie;ZHANG Xinyu;Ainijiang Ersiman;ZHANG Zhidong;WANG Wei;CHU Min(Xinjiang Laboratory of Special Environmental Microbiology,Institute of Microbiology,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,Xinjiang,China;College of Food Sciences and Pharmacy,Xinjiang Agricultural University,Urumqi 830052,Xinjiang,China)

机构地区：[1]新疆农业科学院微生物应用研究所新疆特殊环境微生物实验室,新疆乌鲁木齐830091 [2]新疆农业大学食品科学与药学学院,新疆乌鲁木齐830052

出　　处：《微生物学通报》2024年第8期2857-2869,共13页Microbiology China

基　　金：新疆维吾尔自治区重点研发任务专项(2022B02053-3);新疆农业科学院农业科技创新稳定支持专项(xjnkywdzc-2023005)。

摘　　要：【背景】植物内生菌与宿主存在长期协同演化,是研究微生物多样性和适应性进化的宝库。骆驼刺(Alhagi sparsifolia Shap.)具有耐干旱、耐盐碱等极强的抗逆特性,而有关骆驼刺内生菌的研究较少。【目的】解析骆驼刺可培养内生菌多样性,评价菌株抗逆、促生特性。【方法】采用组织匀浆法和梯度稀释法分离纯化骆驼刺内生菌,利用16S rRNA基因序列比对初步明确菌株的分类定位,进一步测定菌株的抗逆、促生及抑菌功能特性。【结果】从骆驼刺中共分离获得了14株可培养内生细菌,分属于芽孢杆菌属(Bacillus)、短状杆菌属(Brachybacterium)、微杆菌属(Microbacterium)、棒杆菌属(Corynebacterium)、土壤芽孢杆菌属(Solibacillus)、谷氨酸杆菌属(Glutamicibacter)、亮杆菌属(Leucobacter)、短杆菌属(Brevibacterium)及不动杆菌属(Acinetobacter)这9个菌属,其中芽孢杆菌属菌株所占比例最高(33.33%);所获得的菌株表现出不同水平的抗逆、促生及抑菌特性,其中Bacillus subtilis L-1展现出较强的抗逆特性,可耐受20%NaCl及20%的PEG6000胁迫,同时对4种病原细菌及3种病原真菌具有抑菌作用。【结论】骆驼刺内生细菌具有抗逆、促生及抑菌特性,为进一步挖掘和利用相关内生菌资源提供了研究基础和科学依据。[Background]Endophytes represent valuable resources for investigating microbial diversity and adaptive evolution,owing to their long-term co-evolutionary relationship with their hosts.Alhagi sparsifolia Shap.exhibits robust tolerance to stress conditions including drought and saline-alkali.However,the studies about the endophytes of A.sparsifolia remain limited.[Objective]To analyze the diversity of culturable endophytic bacteria and evaluate the stress tolerance and plant growth-promoting effects of the strains.[Methods]Endophytic bacteria were isolated from A.sparsifolia by the tissue homogenization and gradient dilution methods.The strains were identified by comparison of 16S rRNA gene sequences.Subsequently,we determined the stress tolerance,plant growth-promoting effects,and antimicrobial activities of the strains.[Results]A total of 14 culturable endophytic bacterial strains were isolated from A.sparsifolia,belonging to 9 genera(Bacillus,Brachybacterium,Microbacterium,Corynebacterium,Solibacillus,Glutamicibacter,Leucobacter,Brevibacterium,and Acinetobacter),among which Bacillus was dominant(33.33%).These strains exhibited varying levels of stress tolerance,plant growth-promoting effects,and antimicrobial activities.Notably,Bacillus subtilis L-1 demonstrated strong tolerance to 20%NaCl solution and 20%PEG6000 and inhibitory effects on four pathogenic bacterial species and three pathogenic fungal species.[Conclusion]Our study revealed the stress tolerance,plant growth-promoting effects,and antimicrobial activities of culturable endophytic bacterial resources in A.sparsifolia.The findings provide a scientific basis for further exploration and utilization of these valuable resources.

关 键 词：骆驼刺 植物内生菌 抗逆 促生特性 抑菌特性 

分 类 号：Q948.122.3[生物学—植物学]