Secreted Frizzled-Related Protein 5 Mediates Wnt5a Expression in Microcystin-Leucine-Arginine-Induced Liver Lipid Metabolism Disorder in Mice  

作　　者：Meiyan Yang Furong Yu Qianqian Ji Huiying Zhang Jiaxiang Zhang Daojun Chen 

机构地区：[1]Department of Occupational Health and Environmental Health,School of Public Health,Anhui Medical University,Hefei 230032,Anhui,China [2]School of Medical Technology,Anhui Medical College,Hefei 230601,Anhui,China

出　　处：《Biomedical and Environmental Sciences》2024年第8期850-864,共15页生物医学与环境科学（英文版）

基　　金：supported by the Natural Science Research Project of colleges and Universities in Anhui Province[2022AH052336];High Level Talent Research Initiation Fund Of Anhui Medical College[2023RC004]。

摘　　要：Objective Microcystin-leucine-arginine(MC-LR)exposure induces lipid metabolism disorders in the liver.Secreted frizzled-related protein 5(SFRP5)is a natural antagonist of winglesstype MMTV integration site family,member 5A(Wnt5a)and an anti-inflammatory adipocytokine.In this study,we aimed to investigate whether MC-LR can induce lipid metabolism disorders in hepatocytes and whether SFRP5,which has anti-inflammatory effects,can alleviate the effects of hepatic lipid metabolism by inhibiting the Wnt5a/Jun N-terminal kinase(JNK)pathway.Methods We exposed mice to MC-LR in vivo to induce liver lipid metabolism disorders.Subsequently,mouse hepatocytes that overexpressed SFRP5 or did not express SFRP5 were exposed to MC-LR,and the effects of SFRP5 overexpression on inflammation and Wnt5a/JNK activation by MC-LR were observed.Results MC-LR exposure induced liver lipid metabolism disorders in mice and significantly decreased SFRP5 mRNA and protein levels in a concentration-dependent manner.SFRP5 overexpression in AML12cells suppressed MC-LR-induced inflammation.Overexpression of SFRP5 also inhibited Wnt5a and phosphorylation of JNK.Conclusion MC-LR can induce lipid metabolism disorders in mice,and SFRP5 can attenuate lipid metabolism disorders in the mouse liver by inhibiting Wnt5a/JNK signaling.

关 键 词：Jun N-terminal kinase Secreted frizzled-related protein 5 WNT5A Hepatic lipid metabolism disorder 

分 类 号：R575.5[医药卫生—消化系统]