两种菌糠对平菇栽培过程中菌丝生长及产酶能力的影响  

Effects of two kinds of spent mushroom substrate on the growth and enzyme production capacity of Pleurotus ostreatus mycelium during the cultivation time

作　　者：霍心平 武雪娇 高青云 熊翌辰 秦中杰 李奕葶 Huo Xinping;Wu Xuejiao;Gao Qingyun;Xiong Yichen;Qin Zhongjie;Li Yiting(College of Agronomy and Resources Environment,Tianjin Agricultural University,Tianjin 300392,China)

机构地区：[1]天津农学院农学与资源环境学院,天津300392

出　　处：《天津农学院学报》2024年第4期17-22,共6页Journal of Tianjin Agricultural University

基　　金：天津市武清区科技计划项目(WQKJ202123);天津市大学生创新创业训练计划项目(202210061137)。

摘　　要：以白灵菇菌糠和白玉菇菌糠为试验材料,按照不同比例添加到平菇栽培基质中,探讨菌糠对平菇菌丝生长和酶活性的影响。结果表明:添加两种菌糠后,平菇菌丝的生长速度、菌丝长势、满瓶时间等指标均优于对照组;胞外及胞内酶活性测定结果显示,白玉菇菌糠添加量为45%时,羧甲基纤维素酶活性达最高值,为2.61 U/mL;白玉菇菌糠添加量为30%时,木聚糖酶活性最高,为9.02 U/g;白玉菇菌糠添加量为60%时,漆酶活性最高,为263.72 U/L;当栽培料中白灵菇菌糠添加量为60%时,过氧化氢酶活性最高,为13.44 U/g。Using Pleurotus tuoliensis and white Hypsizygus marmoreus spent mushroom substrate as the cultivation medium of Pleurotus ostreatus in different proportions,the study observed the growth and enzyme activity of P.ostreatus mycelium and discussed on the effects of spent mushroom substrate on the growth of P.ostreatus mycelium.The experimental results showed that after adding two types of spent mushroom substrate,the growth rate,mycelial growth potential,and bottle filling time of P.ostreatus mycelium were better than the control group.The carboxymethyl cellulase reached its highest value at 2.61 U/mL when the addition amount of white H.marmoreus spent mushroom substrate was 45%;when the addition amount of white H.marmoreus spent mushroom substrate was 30%,the highest activity of xylanase was 9.02 U/g;the highest laccase activity was 263.72 U/L with 60%white H.marmoreus spent mushroom substrate;the highest catalase activity was 13.44 U/g when the amount of P.tuoliensis spent mushroom substrate was 60%.

关 键 词：菌糠 平菇 栽培基质 菌丝生长 酶活测定 

分 类 号：S646[农业科学—蔬菜学]