粪菌移植对顽固性便秘小鼠的肠道菌群和肠道动力以及TLR4/NF-κB通路蛋白的影响  

Influences of Intestinal Microbiota,Motility and TLR4/NF-kB Signaling Pathway by Fecal Microbiota Transplantation in Mice with Refractory Constipation

作　　者：刘扬[1] 洪文[1] 黄克林 杨博[1] 刘亚坡 路明[1] LIU Yang;HONG Wen;HUANG Ke-lin;YANG Bo;LIU Ya-po;LU Ming(Anorectal Department of the First Affiliated Hospital of Xinjiang Medical University,Urumqi,Xinjiang,830054,China)

机构地区：[1]新疆医科大学第一附属医院肛肠科,新疆乌鲁木齐830054

出　　处：《现代生物医学进展》2024年第16期3020-3024,共5页Progress in Modern Biomedicine

基　　金：新疆维吾尔自治区自然科学基金项目(2021D01C332)。

摘　　要：目的:探讨粪菌移植对顽固性便秘小鼠肠道菌群和肠道动力的影响,是否与Toll样受体4(TLR4)/核转录因子(NF)-κB信号通路有关。方法:健康清洁级C57BL/10小鼠60只,雌雄各半,周龄6~8周,平均体重22 g。随机分为三组,即对照组、模型组和粪菌移植组,各20只。采用灌服盐酸洛哌丁胺(9.38 mg/kg,2次/d,连续6周)的方法复制顽固性便秘模型,评估排便次数和排便量判定造模成功。粪菌移植采用对照组小鼠0.2 m L粪菌液灌肠(2次/d,连续2周)。采用日本光冈肠道菌群分析法定量检测菌群值及B/E值,伊文思蓝推进率法计算肠道动力,Western blot法检测结肠组织TLR4、NF-κB、白细胞介素(IL)-6和肿瘤坏死因子(TNF)-α蛋白表达量。结果:与对照组相比,模型组肠杆菌、肠球菌、拟杆菌和梭菌的菌群值显著升高,B/E值降低;与模型组相比,粪菌移植组酵母菌、双歧杆菌和乳酸杆菌的菌群值明显增加,B/E值升高(P<0.05)。模型组的伊文思蓝推进率显著小于对照组,粪菌移植组显著大于模型组(P<0.05)。模型组TLR4、NF-κB、IL-6和TNF-α蛋白表达量明显高于对照组,而粪菌移植组明显低于模型组(P<0.05)。结论:粪菌移植能够有效改善顽固性便秘小鼠的肠道菌群和肠道动力,可能与下调TLR4/NF-κB信号通路的活性以及抑制肠道组织的炎症反应有关。Objective:To investigate the effect of fecal microbiota transplantation on intestinal microbiota and motility in mice with refractory constipation,whether it was related to Toll like receptor 4(TLR4)/nuclear transcription factor(NF)-κB signal pathway.Methods:60 healthy and clean grade C57BL/10 mice,half of males and females,aged 6~8 weeks,with average weight of 22 g.They were randomly divided into three groups,the control group,model group,and fecal microbiota transplantation group,with 20 mice in each group.The model of refractory constipation was replicated by gavage of loperamide hydrochloride(9.38 mg/kg,twice a day,consecutive for 6 weeks),the number and volume of bowel movements were evaluated to determine the success of model.Fecal microbiota transplantation was performed using 0.2 m L fecal microbiota solution enema of control group mice(twice a day consecutive for 2weeks).Quantitative detection of microbiota value and B/E value were made by the Japanese Guanggang intestinal microbiota analysis method,intestinal motility was calculated by Evans blue propulsion rate,TLR4,NF-κB,interleukin(IL)-6 and tumor necrosis factor(TNF)-αproteins expression in colon tissues was detected by Western blot.Results:Compared with the control group,the bacterial community values of Enterobacterium,Enterococcus,Bacteroides,and Clostridium in the model group significantly increased,while B/E value decreased;compared with the model group,the bacterial community values of yeast,bifidobacteria,and lactobacilli in the fecal microbiota transplantation group significantly increased,while B/E values increased(P<0.05).The Evans blue propulsion rate of the model group was significantly lower than the control group,but it of the fecal microbiota transplantation group was significantly higher than the model group(P<0.05).What's more,the expression levels of TLR4,NF-κB.IL-6 and TNF-α proteins in the model group were significantly higher than the control group,while they were in the fecal microbiota transplantation group were significa

关 键 词：顽固性便秘 粪菌移植 肠道菌群 肠道动力 Toll样受体4 核转录因子-ΚB 

分 类 号：R-33[医药卫生] R442.2