Long noncoding nuclear enriched abundant transcript 1_2 is a promising biomarker for childhood-onset systemic lupus erythematosus  

作　　者：Shipeng Li Xia Wang Xiaozhen Zhao Jianghong Deng Weiying Kuang Junmei Zhang Xiaohua Tan Chao Li Caifeng Li 

机构地区：[1]Department of Rheumatology,Beijing Children's Hospital,Capital Medical University,National Center for Children's Health,Beijing,China [2]Center for Infectious Diseases,Beijing Youan Hospital,Capital Medical University,Beijing Key Laboratory for HIV/AIDS Research,Beijing,China

出　　处：《Pediatric Investigation》2024年第2期101-107,共7页儿科学研究（英文）

基　　金：National Natural Science Foundation of China,Grant/Award Number:81701604;R&D program of Beijing Municipal Education Commission,Grant/Award Number:202210025037。

摘　　要：Importance:Systemic lupus erythematosus(SLE)is a diffuse connective tissue disease with complex clinical manifestations and prolonged course.The early diagnosis and condition monitoring of SLE are crucial to disease prognosis.Objective:To assess the diagnostic value of long noncoding RNA(lncRNA)nuclear enriched abundant transcript 1(NEAT1)in childhood-onset SLE(cSLE).Methods:Fifty-seven children diagnosed with SLE,40 children diagnosed with juvenile idiopathic arthritis(JIA),and 40 healthy children were included.Peripheral blood samples from each patient were collected.A quantitative polymerase chain reaction was used to confirm the expression of lncNEAT1_1 and lncNEAT1_2 in peripheral blood.Associations among parameters were analyzed using the Mann-Whitney U test or independent sample t-test.Results:The expression of both lncNEAT1_1 and lncNEAT1_2 in patients with cSLE were significantly higher than that of healthy control and patients with JIA.Receiver operating characteristic curves revealed an area under the curve(AUC)of 0.633(95%confidence interval[CI],0.524-0.742;P=0.024)for lncNEAT1_1.The AUC of lncNEAT1_2 was 0.812(95%CI,0.727-0.897;P<0.0001)to discriminate individuals with cSLE from health control and children with JIA with a sensitivity of 0.622 and a specificity of 0.925.Moreover,lncNEAT1_2 expression was higher in patients with cSLE presenting with fever,lupus nephritis,elevated erythrocyte sedimentation rate,active disease activity,and decreased C3 level,compared with those without these conditions.However,no similar correlation was observed for lncNEAT1_1.Interpretation:The expression of lncNEAT1_2 was significantly elevated in children with SLE,especially those with fever,renal involvement,and low C3 levels.These findings suggest that lncNEAT1_2 may represent a potential biomarker for cSLE.

关 键 词：BIOMARKER Children LncRNA SLE NEAT1 

分 类 号：R725.9[医药卫生—儿科]